Microscope stage cell culture incubator

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Overview

- 3D-printed incubation chamber for cell culture in microfluidic chips
- minimized gas consumption and evaporation
- access and organization of many tubes for e.g. microfluidics control

- optimized for inverted microscopes
- cheap
- easy to produce and use

Download Files

DownloadIncubation Chamber (ZIP, 3.5 MB)

.zip Contents:

STL file for standard slicer software:
- Incubator_V13.stl: 3D printable incubator

Laser cutting templates:
- Chip_Plate_V3.pdf: transparent incubator bottom plate, 2mm thick transparent PMMA
- Plate_5mm.pdf: lid frame, 5mm thick transparent PMMA
- Rubber_Gasket_pt2.pdf: lid gasket, 2mm thick natural rubber
- Window_2mm_V2.pdf: lid, 2mm transparent PMMA

- Incubation Chamber Setup.docx: Assembly instructions
 

References

If you use the incubation chamber please cite the following paper:
Dettinger, Wang, Ahmed, Zhang, Loeffler, Kull, Etzrodt, Lengerke and Schroeder (2020)
external pageAn Automated Microfluidic System for Efficient Capture of Rare Cells and Rapid Flow-free Stimulation
Lab on a Chip
, 10.1039/D0LC00687D

 

License

Copyright (c) 2020 Schroeder Lab

Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:

The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software.

THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.

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