Stand-Alone & Flex Automation Systems
We also offer a number of stand-alone systems which can be used in isolation or linked using robotic arms and custom software developed in house.
Immersion Cycler
High-throughput system capable of handling up to 20x 384-well plates in parallel for temperature or enzamatically controlled reactions. Currently set up for Golden Gate Assemblies.
For more information, please visit our wiki.
Immunofluorescence Staining Protocol
Using an Opentrons-OT2 to perform the following protocol.
- Remove excess liquid
- PBS wash 3x
- Add & remove permeabilization solution
- Add & remove blocking solution
- Add primary antibodies and incubate overnight
- PBS wash 3x
- Add secondary antibodies solution
- BPS wash 3x
- Add & remove DAPI
- PBS wash 3x
- Add PBS + Sodium Azide
Protocol and Python codebase available upon request. For more information, please visit our wiki.
Periodic measurements every 30 minutes over a 4 day period
- Incubate 96-well plates at 37 °C
- Monitor growth via absorbance at 600 nm
- Measure fluorescence
- De-lid plate prior to measurement
- Run multiple plates in parallel
For more information, please visit our wiki.
For more information, see the list of equipment in the facility, or enquire directly about possible custom solutions.
More information about protocols, workflows, and projects under development can be found on our wiki.
Contact LAF for services, location, and information requests
Department of Biosystems Science and Engineering