tTt and qTfy
The Tracking Tool (tTt) and qTfy enable single-cell tracking and quantification of cellular and molecular properties in time-lapse imaging data. tTt allows efficient and user-friendly manual cell tracking, including the possibility to import, inspect and correct existing tracked trees (e.g. from auto-tracking tools). qTfy extends the tracked trees exported by tTt with fluorescence measurements to generate quantitative single-cell genealogies. Both tools allow robust and efficient analysis of large amounts of imaging data and are not limited to specific cell or image types.
Instructions
Download short guides how to use tTt and qTfy:
tTt, qTfy and tTt Converter
Download automatic installers for Windows (use right-click and save as):
Download tTt v3.4.4, qTfy v1.0.0 and tTt Converter - Windows 64 Bit (MSI, 16.3 MB)
[alternative download link]
Download tTt v3.4.4, qTfy v1.0.0 and tTt Converter - Windows 32 Bit (MSI, 14.4 MB)
[alternative download link]
Example data
Download Download example dataset (ZIP, 225.7 MB)
[alternative download link]
Source Code
The C++ source code for tTt, qTfy and tTt Converter can be found in our external page GitLab.com repository
References
If you use tTt, qTfy or tTt Converter for your research, please cite the following paper:
Hilsenbeck O*, Schwarzfischer M*, Skylaki S*, Schauberger B, Hoppe PS, Loeffler D, Kokkaliaris KD, Hastreiter S, Skylaki E, Filipczyk A, Strasser M, Buggenthin F, Feigelman JS, Krumsiek J, van den Berg AJJ, Endele M, Etzrodt M, Marr C, Theis FJ* and Schroeder T* (2016)
Software tools for single-cell tracking and quantification of cellular and molecular properties
Nature Biotechnology, 34: 703-706
License
tTt, qTfy and tTt Converter are free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.
This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details (external page http://www.gnu.org/licenses/gpl-3.0.html).