Journal Articles

2024

Monitoring the mass, eigenfrequency, and quality factor of mammalian cells

S. Herzog, G. Fläschner, I. Incaviglia, J. Casares Arias, A. Ponti, N. Strohmeyer, M.M. Nava & D.J. Müller

Nature Communications (2024) 15, 1751. external page Read more.

Light Color-Controlled pH-Adjustment of Aqueous Solutions Using Engineered Proteoliposomes

D. Harder, N. Ritzmann, Z. Ucurum, D.J. Müller & D. Fotiadis
Advanced Science (2024) in press. external page Read more

Mechanical stimulation and electrophysiological monitoring at sub-cellular resolution reveals differential mechanosensation of neurons within networks

K.C .Kasuba, A.P. Buccino, J. Bartram, B.M. Gaub, F.J. Fauser, S. Ronchi, M.A. Nava, A. Hierlemann & D.J. Müller
Nature Nanotechnology (2024) in press. external page Read more.

2023

The insertase YidC chaperones the polytopic membrane protein MelB inserting and folding simultaneously from both termini

N. Blaimschein, H. Parameswaran, G. Nagler, A. Kuhn, L. Guan & D.J. Müller
Structure (2023) 31, 1419-1430.

Reply to: Antibiotics and hexagonal order in the bacterial outer membrane

S. Manioglu, S.M. Modaresi, J. Thoma, S.A. Overall, G. Upert, A. Luther, A.B. Barnes, D. Obrecht, D.J. Müller & S. Hiller
Nature Communications (2023) 14, 4773.

Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi

S. Yaganoglu, K. Kalyviotis, C. Vagena-Pantoula, D. Jülich, N. Helassa, B.M. Gaub, M. Welling, T. Lopes, D. Lachowski, S.S. Tang, A. del Río Hernández, V. Salem, D.J. Muller, S. Holley, J. Vermot, J. Shi, N. Helassa, K. Török & P. Pantazis
Nature Communications (2023) 14, 4352.

The CRL4B E3 ubiquitin ligase complex regulates mitosis and brain development by recruiting phospho-specific DCAFs

A. Stier, S. Gilberto, W. Mohamed, L.N. Royall, J. Helenius, I. Mikicic, T. Sajic, P. Beli, D.J. Müller, S. Jessberger & M. Peter
EMBO Journal (2023) e112847. external page Read more.

Engineered biomimetic fibrillar fibronectin matrices regulate cell adhesion initiation, migration, and proliferation via α5β1 integrin and syndecan-4 crosstalk

S. Ahn, U. Sharma, K.C. Kasuba, N. Strohmeyer & D.J. Müller
Advanced Science (2023) 10, 2370163. external page Read more.

Structural basis for ninjurin-1 mediated plasma membrane rupture in cell death

M. Degen, J.C. Santos, K. Pluhackova, G. Cebrero, S. Ramos, G. Jankevicius, E. Hartenian,U. Guillerm, S.A. Mari, B. Kohl, D.J. Müller, P. Schanda, T. Maier, C. Perez, C. Sieben, P. Broz & S. Hiller
Nature (2023) 618, 1065-1071. external page Read more.

In mitosis integrins reduce adhesion to extracellular matrix and strengthen adhesion to adjacent cells

M. Huber, J. Casares-Aires, R. Fässler, D.J. Müller & N. Strohmeyer
Nature Communications (2023) 14, 2143. external page Read more.

Tailoring the Sensitivity of Microcantilevers To Monitor the Mass of Single Adherent Living Cells

I. Incaviglia, S. Herzog, G. Fläschner, N. Strohmeyer, E. Tosoratti & D.J. Müller
Nano Letters (2023) 23, 588–596. external page Read more.

Substrate-binding guides individual melibiose permeases MelB to structurally soften and to destabilize cytoplasmic middle-loop C3

N. Blaimschein, P. Hariharan, S. Manioglu, L. Guan & D.J. Müller
Structure (2023) 31, 58-67. external page Read more.

2022

Antibiotic polymyxin arranges lipopolysaccharide into crystalline structures to solidify the bacterial membrane

S. Manioglu, S.M. Modaresi, N. Ritzmann, J. Thoma, S. Overall, A. Harms, G. Upert, A. Luther, A. Barnes, D. Obrecht, D.J. Müller & S. Hiller
Nature Communications (2022) 13, 6195. external page Read more.

Synthetic Biology: Bottom-Up Assembly of Molecular Systems

S. Hirschi, T.R. Ward, W. Meier, D.J. Müller & D. Fotiadis
Chemical Reviews (2022) 122, 16294–16328. external page Read more.

High-resolution mass measurements of single budding yeast reveal linear growth segments

A.P. Cuny, K.T. Sapra, D. Martinez-Martin, G. Fläschner, J.D. Adams, S. Martin, Ch. Gerber, F. Rudolf & D.J. Müller
Nature Communications (2022) 13, 3483. external page Read more.

A cholesterol analog stabilizes the human β2-adrenergic receptor nonlinearly with temperature

T. Serdiuk, M. Manna, C. Zhang, S.A. Mari, W. Kulig, K. Pluhackova, B.K. Kobilka, I. Vattulainen & D.J. Müller
Science Signaling (2022) 15, eabi7031. external page Read more.

Gasdermin-A3 pore formation propagates along variable pathways

S.A. Mari, K. Pluhackova, J. Pipercevic, M. Leipner, S. Hiller, A. Engel & D.J. Müller
Nature Communications (2022) 13, 2609. external page Read more.

Monitoring the antibiotic darobactin modulating the beta-barrel assembly factor BamA

N. Ritzmann, S. Manioglu, S. Hiller & D.J. Müller
Structure (2022) 30, 350-359. external page Read more.

2021

Lipids and phosphorylation conjointly modulate complex formation of β2-adrenergic receptor and βarrestin-2

K. Pluhackova, F. Wilhelm & D.J. Müller
Frontiers in Cell and Developmental Biology (2021) 9, 807913. external page Read more.

Monitoring the binding and insertion of a single transmembrane protein by an insertase

P.R. Laskowski, K. Pluhackova, M. Haase, B.M. Lang, G. Nagler, A. Kuhn & D.J. Müller
Nature Communications (2021) 12, 7082. external page Read more.

Force spectroscopy of single cells using atomic force microscopy

A. Viljoen, M. Mathelié- Guinlet, A. Ray, N. Strohmeyer, Y.J. Oh, P. Hinterdorfer, D.J. Müller, D. Alsteens & Y.F. Dufrêne
Nature Methods Reviews (2021) 1 , 63. external page Read article.

Proton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells

K. Jahnke, N. Ritzmann, J. Fichtler, A. Nitschke, Y. Dreher, T. Abele, G. Hofhaus, I. Platzman, R. Schroder, D.J. Müller, J. Spatz & K. Göpfrich
Nature Communications (2021) 12, 3967. external page Read article.

Rheology of rounded mammalian cells over continuous high-frequencies

G. Fläschner, C.I. Roman, N. Strohmeyer, D. Martinez & D.J. Müller
Nature Communications (2021) 12, 2922. external page Read article.

Scanning probe microscopy

K. Bian, C. Gerber, A.J. Heinrich, D.J. Müller, S. Scheuring & Y. Jiang
Nature Methods Reviews (2021) 1, 36. external page Read article.

Atomic force microscopy-based force spectroscopy and multiparametric imaging of biomolecular and cellular systems

D.J. Müller, A.C. Dumitru, C. Lo Giudice, H.E. Gaub, P. Hinterdorfer, G. Hummer, J. De Yoreo, Y. Dufrêne YF & D. Alsteens
Chemical Reviews (2020) 121, 11701-11725.

2020

Nonlinear mechanics of lamin filaments and the meshwork topology build an emergent nuclear lamina

K.T. Sapra, Z. Quin, U. Aebi, D.J. Müller, M. Buehler & O. Medalia
Nature Communications (2020) 11, 6205. external page Read article.

Binding of αv-class integrins to fibronectin is exclusively mediated by the Arg-Gly-Asp (RGD) motif and unaffected by an Asp to Glu substitution (RGE)

M. Benito-Jardón, N. Strohmeyer, S. Ortega-Sanchís, M. Bharadwaj, M. Moser, D.J. Müller, R. Fässler & M. Costell
Journal of Cell Biology (2020) 219, e202004198. external page Read article.

The nucleus acts as a ruler tailoring cell responses to spatial constraints

A. Lomakin, C.J. Cattin, D. Cuvelier, Z. Alraies, M. Molina, G.P.F. Nader, N. Srivastava, P.J. Saez, J.M. Garcia-Arcos, I.Y. Zhitnyak, A. Bhargava, M.K. Driscoll, E.S. Welf, R. Fiolka, R.J. Petrie, N.S. De Silva, J.M. González-Granado, N. Manel, A.M. Lennon-Duménil, T. Kraushaar, R. Hartmann, D. Hoffmann, E. Jelli, K. Descher, D.J. Müller, & M. Piel
Science (2020) 370, eaba2894. external page Read article. external page Read News & Views.

Kin discrimination in social yeast is mediated by cell surface receptors of the Flo11 adhesin family

S. Brückner, R. Schubert, T. Kraushaar, R. Hartmann, D. Hoffmann, E. Jelli, K. Descher, D.J. Müller, L.O. Essen & H.U. Mösch
eLife (2020) 9, e55587. external page Read article.

Micelle formation among various mechanisms of toxin pore formation

Martin Vögele, Ramachandra M. Bhaskara, E. Mulvihill, K. van Pee, Ö. Yildiz, W. Kühlbrandt, D.J. Müller & G. Hummer
Proc. Natl. Acad. Sci. USA (2020) 117, 5109-5110.  

Protease-activated receptors use multiple pathways to activate α5β1 integrins and assemble adhesomes in non-hematopoietic cells

P.M. Spoerri, N. Strohmeyer, Z. Sun, R. Fässler & D.J. Müller
Nature Materials (2020) 9, 218-226. external page Read article.

Neurons differentiate magnitude and location of mechanical stimuli

B.M. Gaub, K. Kasuba Chaitanya, E. Mace, T. Strittmatter, P.R. Laskowski, S.A. Geissler, A. Hierlemann, M. Fussenegger, B. Roska & D.J. Müller
Proc. Natl. Acad. Sci. USA (2020) 117, 848-856. external page Read article.

2019

High-resolution imaging of maltoporin LamB while quantifying the free-energy landscape and asymmetry of sugar-binding

E. Mulvihill, M. Pfreundschuh, J. Thoma, N. Ritzmann & D.J. Müller
Nano Letters (2019) 19, 6442-6453. external page Read article.

Conformational plasticity of the human protease-activated receptor 1 upon antagonist- and agonist-binding

P.M. Spoerri, K.T. Sapra, C. Zhang, S.A. Mari, H. Kato, B.K. Kobilka & D.J. Müller
Structure (2019) 27, 1517-1526. external page Read article.

Magnetically guided virus stamping for the targeted infection of single cells or groups of cells

R. Schubert, S. Herzog, S. Trenholm, B. Roska & D.J. Müller
Nature Protocols (2019) 14, 3205-3219. external page Read article.

Membrane perforation by the pore-forming toxin pneumolysin

M. Vögele, R.M. Bhaskara, E. Mulvihill, K. van Pee, Ö. Yildiz, W. Kühlbrandt, D.J. Müller & G. Hummer
Proc. Natl. Acad. Sci. USA (2019) 116, 13352-13357. external page Read article.

Spatiotemporally controlled myosin relocalization and internal pressure generate sibling cell size asymmetry

T.T. Pham, A. Monnard, J. Helenius, E. Lund, N. Lee, D.J. Müller & C. Cabernard
iScience (2019) 13, 9-19. external page Read article.

Design and assembly of a chemically switchable and fluorescently traceable light-driven proton pump system for bionanotechnological applications

S. Hirschi, N. Fischer, D. Kalbermatter, P.R. Laskowski, Z. Ucurum, D.J. Müller & D. Fotiadis
Scientific Reports (2019) 9, 1046. external page Read article.

Insertion and folding pathways of single membrane proteins guided by translocases and insertases

T. Serdiuk, A. Steudle, S.A. Mari, S. Manioglu, H.R. Kaback, A. Kuhn & D.J. Müller
Science Advances (2019) 5, eaau6824. external page Read article.

Seeing and sensing single G-protein coupled receptors by atomic force microscopy

T. Sapra, P. Spoerri, A. Engel, D. Alsteens & D.J. Müller
Current Opinion Cell Biology (2019) 57, 25-32. external page Read article.

Vaccinia virus hijacks EGFR signalling to enhance virus spread through rapid and efficient infected cell motility

C. Beerli, A. Yakimovich, S. Kilcher, G.V. Reynoso, G. Fläschner, D.J. Müller, H.D. Hickman & J. Mercer
Nature Microbiology (2019) 4, 216-225 external page Read article.

Atomic force microscopy-based mechanobiology

M. Krieg, G. Fläschner, D. Alsteens, B.M. Gaub, W.H. Roos, G.J.L. Wuite, H.E. Gaub, C. Gerber, Y.F. Dufrêne & D.J. Müller
Nature Reviews Physics (2019) 1, 41-57. external page Read article.

2018

Optimized reconstitution of membrane proteins into synthetic membranes

R. Goers, J. Thoma, N. Ritzmann, A. Di Silvestro, C. Alter, G. Gunkel-Grabole, D. Fotiadis, D.J. Müller & W. Meier
Communications Chemistry (2018) 1, 35. external page Read article.

Oscillatory switches of dorso-ventral polarity in cells confined between two surfaces

J. Helenius, M. Ecke, D.J. Müller & G. Gerisch
Biophysical Journal (2018) 15, 150-162. external page Read article.

Imaging in biologically-relevant environments with AFM using stiff qPlus sensors

K. Pürckhauer, A.J. Weymouth, K. Pfeffer, L. Kullmann, E. Mulvihill, M.P. Krahn, D.J. Müller & F.J. Giessibl
Scientific Reports (2018) 8, 9330. external page Read article.

Mechanism of membrane pore formation by human Gasdermin-D

E. Muvihill, L. Sborgi, S.A. Mari, M. Pfreundschuh, S. Hiller & D.J. Müller
EMBO Journal (2018) 37, e98321. external page Read article. Read external page News & Views.external page

Cells stiffen for cytokines

R. Newton & D.J. Müller
Cell Chemical Biology (2018) 25, 495-496. external page Read article.

POTRA-domains, extracellular lid and membrane composition modulate the conformational stability of the beta-barrel assembly factor BamA

J. Thoma, Y. Sun, N. Ritzmann & D.J. Müller
Structure (2018) 26, 987-996. external page Read article.

Reversible cation-selective attachment and self-assembly of human tau on supported brain lipid membranes  

S.A. Mari, S. Wegmann, K. Tepper, B. Hyman, E.M. Mandelkow, E. Mandelkow & D.J. Müller
Nano Letters (2018) 18, 3271-3281. external page Read article.

Structural properties of the human protease-activated receptor 1 changing by a strong antagonist

P. Spoerri, H.E. Kato, M. Pfreundschuh, S.A. Mari, T. Serdiuk, J. Thoma, K.T. Sapra, C. Zhang, B.K. Kobilka & D.J. Müller
Structure (2018) 26, 829-838. external page Read article.

Single-molecule force spectroscopy of transmembrane β-barrel proteins

J. Thoma, K.T. Sapra & D.J. Müller
Annual Reviews of Analytical Chemistry (2018) 11, 375-395. external page Read article.

Protein-enriched outer membrane vesicles as a native platform for outer membrane protein studies

J. Thoma, S. Manioglu, D. Kalbermatter, P. Bosshart, D. Fotiadis & D.J. Müller
Communications Biology (2018) 1, 23. external page Read article.

Tau protein liquid-liquid phase separation can initiate tau aggregation

S. Wegmann, B. Eftekharzadeh, K. Tepper, K.M. Zoltowska, R.E. Bennett, S. Durjadin, P.R. Laskowski, D. MacKenzie, T. Kamath, C. Commins, C. Vanderurg, A.R. Roe, Z. Fan, A.M. Molliex, A. Hernandez-Varga, D.J. Muller, A.A. Hyman, E. Mandelkow, & B.T. Hyman
EMBO Journal (2018) 37, e98049. external page Read article.

Virus stamping for targeted single cell infection in vitro and in vivo

R. Schubert, S. Trenholm, K. Balint, G. Kosche, C.S. Cowan, M.A. Mohr, M. Munz, D. Martinez-Martin, G. Fläschner, R. Newton, J. Krol, B. Gross Scherf, K. Yonehara, A. Wertz, A. Ponti, A. Ghanem, D. Hillier, K. Conzelmann, D.J. Müller & B. Roska
Nature  Biotechnology (2018) 36, 81-88. external page Read article and reports external page 1 & external page 2.

2017

Fibronectin-bound α5β1 integrins sense load and signal to reinforce adhesion in less than a second

N. Strohmeyer, M. Bharadwaj, M. Costell, R. Fässler & D.J. Müller
Nature  Materials (2017) 12, 1262-1270. external page Read article and external page report.

Inertial picobalance reveals fast mass fluctuations of mammalian cells

D. Martínez-Martín, G. Fläschner, B. Gaub, S. Martin, R. Newton, C. Beerli, J. Mercer, C. Gerber & D.J. Müller
Nature  (2017) 550, 500-505. external page Read article, reports external page 1 & external page 2 and external page Nature Award for the best Science Images in 2017.

Genome-scale single-cell mechanical phenotyping reveals disease-related genes involved in mitotic rounding

Y. Toyoda, C.J. Cattin, M.P. Stewart, I. Poser, M. Theis, T.V. Kurzchalia, F. Buchholz, A.A. Hyman & D.J. Müller
Nature Communications (2017) 8, 1266. external page Read More.

Combining confocal and atomic force microscopy to quantify single-virus binding to mammalian cell surfaces

R. Newton, M. Delguste, M. Kohler, A.C. Dumitru, P. Laskowski, D.J. Müller & D. Alsteens
Nature Protocols (2017) 12, 2275-2292. external page Read More.

High-resolution imaging and multiparametric characterization of native membranes by combining confocal microscopy and an atomic force microscopy-based toolbox

P.R. Laskowski, M. Pfreundschuh, M. Stauffer, Z. Ucurum, D. Fotiadis & D.J. Müller
ACS Nano (2017) 11, 8292-8301. external page Read More.

Fusion domains guide the oriented insertion of light-driven proton pumps into liposomes

N. Ritzmann, J. Thoma, S. Hirschi, D. Kalbermatter, D. Fotiadis & D.J. Müller 
Biophysical Journal (2017) 113, 1181-1186. external page Read More.

Pull-and-paste of single transmembrane proteins

T. Serdiuk, S.A. Mari & D.J. Müller 
Nano Letters (2017) 17, 4478-4488. external page Read More.

Maltoporin LamB unfolds beta-hairpins along mechanical stress-dependent unfolding pathways

J. Thoma, N. Ritzmann, D. Wolf, E. Mulvihill, S. Hiller & D.J. Müller 
Structure (2017) 25, 1139-1142. external page Read More.

Atomic force microscopy imaging modalities in molecular and cell biology

Y.F. Dufrêne, T. Ando, R. Garcia, D. Alsteens, D. Martinez-Martin, A. Engel, C. Gerber & D.J. Müller 
Nature Nanotechnology  (2017) 12, 295-307. external page Read More.

Detecting ligand-binding events and free energy landscape while imaging membrane receptors at sub-nanometer resolution

M. Pfreundschuh, D. Harder, Z. Ucurum, D.C. Fotiadis & D.J. Müller 
Nano Letters (2017) 17, 3261-3269. external page Read More.

Atomic force microscopy-based characterization and design of biointerfaces

D. Alsteens, H.E. Gaub, R. Newton, M. Pfreundschuh, C. Gerber & D.J. Müller 
Nature Reviews Materials (2017) 2, 17008. external page Read More.

Membrane proteins scrambling through a folding landscape

D.J. Müller & H.E. Gaub
Science (2017) 355, 907-908. external page Read More.

Mechanical stimulation of Piezo1 receptors depends on extracellular matrix proteins and directionality of force

B.M. Gaub & D.J. Müller 
Nano Letters (2017) 17, 2064-2072. external page Read More.

Nanomechanical mapping of first binding steps of a virus to animal cells

D. Alsteens, R. Newton, R. Schubert, D. Martinez-Martin, M. Delguste, B. Roska & D.J. Muller 
Nature Nanotechnology (2017) 12, 177-183. external page Read More.

The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes

M Benito-Jardón, S. Klapproth, I. Gimeno-LLuch, T. Petzold, M. Bharadwaj, D.J. Müller, G. Zuchtriegel, C.A. Reichel, M. Costell 
eLife (2017) 6, 22264. external page Read More.

αV-class integrins exert dual roles on α5β1 integrins to strengthen adhesion to fibronectin

M. Bharadwaj, N. Strohmeyer, G.P. Colo, J. Helenius, N. Beerenwinkel, H.B. Schiller, R. Fässler & D.J. Müller
Nature Communications (2017) 8, 14348. external page Read More.

2016

Mechanism of allosteric regulation of β2-adrenergic receptor by cholesterol

M. Manna, M. Niemelä, J. Tynkkinen, M. Javanainen, W. Kulig, D.J. Müller, T. Rog & I. Vattulainen
eLife (2016) 5, e18432.external page Read More.

Unraveling the pore forming steps of pneumolysin from Streptococcus pneumoniae  

K. van Pee, E. Mulvihill, D.J. Müller & Ö. Yildiz
Nano Letters (2016) 6, 7915-7924. external page Read More.

The biomechanical properties of an epithelial tissue determine the location of its vasculature

M. Kragl, R. Schubert, H. Karsjens, S. Otter, B. Bertosinska, K. Jeruschke, J. Weiss, C. Chen,  D. Alsteens, O. Kuss, S. Speier, D. Eberhard, D.J. Müller & E. Lammert 
Nature Communications (2016) 7, 13560. external page Read More.

YidC assists the stepwise and stochastic folding of membrane proteins

T. Serdiuk, D. Balasubramaniam, J. Sugihara, S.A. Mari, H.R. Kaback & D.J. Muller 
Nature Chemical Biology (2016) 12, 911-917. external page Read More

Engineering a chemical switch into the light-driven proton pump proteorhodopsin by cysteine mutagenesis and thiol modification

D. Harder, S. Hirischi, Z. Ucurum, R. Goers, W. Meier, D.J. Müller & D. Fotiadis  
Angewandte Chemie International Edition (2016) 55, 8846-8849. external page Read More

GSDMD membrane pore formation constitutes the mechanism of pyroptotic cell death

L. Sborgi, S. Rühl, E. Mulvihill, J. Pipercevic, R. Heilig, H. Stahlberg, C.J. Farady, D.J. Muller, P. Broz & S. Hiller
EMBO Journal (2016) 35, 1766-1778. external page Read More

Rheology of the active cell cortex in mitosis

E. Fischer-Friedrich, Y. Toyoda, C.J. Cattin, D.J. Müller, A.A. Hyman & F. Jülicher 
Biophysical Journal (2016) 11, 589-600. external page Read More

Engineering and assembly of protein modules into functional systems

S. Hirschi, U. Stauffer, D. Harder, D.J. Müller, W. Meier & D. Fotiadis 
Chimia (2016) 70, 398-401. external page Read More

A glucose-starvation response regulates the diffusion of macromolecules

R.P. Joyner, J.H. Tang, J. Helenius, E. Dultz, C. Brune, L.J. Holt, S. Huet, D.J. Müller & K. Weis
eLife (2016) 5, e09376. external page Read Mole

Molecular plasticity of the human voltage-dependent anion channel embedded into a membrane

L. Ge, S. Villinger, S.A. Mari, K. Giller, C. Griesinger, S. Becker, D.J. Müller & M. Zweckstetter 
Structure (2016) 24, 585-594. external page Read More

Monitoring backbone hydrogen bond formation in β-barrel membrane protein folding

Raschle, P. Rios Flores, C. Opitz, D.J. Müller & S. Hiller
Angewandte Chemie International Edition (2016) 55, 5952-5955. external page Read More

SAS-6 engineering reveals interdependency between cartwheel and microtubule wall in determining centriole architecture

M. Hilbert, A. Noga, D. Frey, V. Hamel, P. Guichard, S.H.W. Kraatz, M. Pfreundschuh, S. Hosner, I. Flückiger, R. Jaussi, M.M. Wieser, K.M. Thieltges, X. Deupi, D.J. Müller, R.A. Kammerer, P. Gönczy, M. Hirono & M.O. Steinmetz
Nature Cell Biology (2016) 18, 393-403. external page Read More

Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin

M. Theodosiou, M. Widmaier, R.T. Böttcher, E. Rognoni, M. Veelders, M. Bharadwaj, A. Lambacher, K. Austen, D.J. Müller, R. Zent & R. Fässler
eLife (2016) 5, e10130. external page Read More

2015

How to minimize artifacts in atomistic simulations of membrane proteins, whose crystal structure is heavily engineered: β2-adrenergic receptor in the spotlight

M. Manna, W. Kulig, M. Javanainen, J. Tynkkynen, U. Hensen, D.J. Müller, T. Rog & I. Vattulainen
Journal of Chemical Theory and Computation (2015) 11, 3432-3445. external page Read More

Quantifying and identifying two ligand-binding sites while imaging native human  membrane receptors by AFM

M. Pfreundschuh, D. Alsteens, R. Wieneke, C. Zhang, S.R. Coughlin, R. Tampé, B.K. Kobilka & D.J. Müller
Nature Communications (2015) 6, 8857. external page Read More

Mitotic cells contract actomyosin cortex and generate pressure to round up against or escape epithelial confinement

B. Sorce, C. Escobedo, Y. Toyoda, M.P. Stewart, C.J. Cattin, R. Newton, I. Banerjee, A. Stettler, B. Roska, S. Eaton, A.A. Hyman, A. Hierlemann & D.J. Müller 
Nature Communications (2015) 6, 8872. external page Read More

Directly observing the lipid-dependent self-assembly and pore-forming mechanism of the cytolytic toxin listeriolysin O

E. Mulvihill, K. van Pee, S.A. Mari, D.J. Müller & Ö. Yildiz
Nano Letters (2015) 15, 6965-6973 external page Read More

Mechanical control of mitotic progression in single animal cells

C.J. Cattin, M. Düggelin, D. Martinez-Martin, C. Gerber, D.J. Müller & M.P. Stewart
Proc. Natl. Acad. Sci. USA (2015), 112, 11258-11263. external page Read More

Impact of holdase chaperones Skp and SurA on the folding of β-barrel outer membrane proteins

J. Thoma, B.M. Burmann, S. Hiller & D.J. Muller
Nature Structural and Molecular Biology (2015), 22, 795-802. external page Read More

Neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau species derived from tau-transgenic mouse and human Alzheimer’s disease brain

S. Takeda, S. Wegmann, H. Cho, C. Commins, A.D. Roe, S.B. Nicholls, G.A. Carlson, R. Pitstick, C.K. Nobuhara, I. Costantino, M.P. Frosch, D.J. Müller, D. Irimia & B.T. Hyman
Nature Communications (2015), 6, 8490. external page Read More

Imaging G protein-coupled receptors while quantifying their ligand-binding free-energy landscape

D. Alsteens, M. Pfreundschuh, C. Zhang, P. Spoerri, S.R. Coughlin, B.K. Kobilka & D.J. Müller
Nature Methods (2015) 12, 845-851. external page Read More

Single-molecule force spectroscopy of membrane proteins from membranes freely spanning across nanoscopic pores

R. Petrosyan, C.A. Bippes, S. Walheim, D. Harder, D. Fotiadis, T. Schimmel, D. Alsteens & D.J. Müller
Nano Letters (2015) 15, 3624-3433. external page Read More

Dynamic single-molecule force spectroscopy of rhodopsin in native membranes

P.S. Park & D.J. Muller
Methods in Molecular Biology (2015) 1271, 173-185. external page Read More

Observing a lipid-dependent alteration in single lactose permeases

T. Serdiuk, J. Sugihara, S.A. Mari, H.R. Kaback & D.J. Müller
Structure (2015) 23, 754-761. external page Read More

In PC3 prostate cancer cells ephrin receptors crosstalk to beta1-integrins to strengthen adhesion to collagen type I

M. Yu, J. Wang, D.J. Muller & J. Helenius
Scientific Reports (2015) 5, 8206. external page Read More

Action of the Hsp70 chaperone system observed with single proteins

J.M. Nunes, M. Mayer-Hartl, F.U. Hartl & D.J. Muller
Nature Communications (2015) 6, 6307. external page Read More

Cdk1 dependent mitotic enrichment of cortical myosin II promotes cell rounding against confinement

S.P. Ramanathan, J. Helenius, C. Cattin, A.A. Hyman, M.P. Stewart & D.J. Muller
Nature Cell Biology (2015) 17, 148-159. external page Read More

Toward high-throughput biomechanical phenotyping of single molecules

D. Alsteens, S. Tay & D.J. Muller
Nature Methods (2015) 12, 45-46. external page Read More

2014

Oligomer formation of Tau protein hyperphosphorylated in cells

K. Tepper, J. Biernat, S. Kumar, S. Wegmann, T. Timm, S. Hübschmann, L. Redecke, E.M. Mandelkow, D.J. Müller & E. Mandelkow
Journal of Biological Chemistry (2014) 289, 34389-343407. external page Read More

Quantification of surface tension and internal pressure generated by single mitotic cells

E. Fischer-Friedrich, A.A. Hyman, F. Jülicher, D.J. Muller & J. Helenius
Scientific Reports (2014) 4, 6213. external page Read More

Products of the Parkinson's-disease related glyoxalase DJ-1, D-lactate and glycolate, support mitochondrial membrane potential and neuronal survival

Y. Toyoda, C. Erkut, F. Pan-Montojo, S. Boland, M.P. Stewart, D.J. Müller, W. Wurst, A.A. Hyman & T.V. Kurzchalia
Biology Open (2014) 3, 777-784. external page Read More

Assay for characterizing the recovery of vertebrate cells for adhesion measurements by single-cell force spectroscopy

R. Schubert, N. Strohmeyer, M. Bharadwaj, R. Subramaniam, M. Krieg, J. Friedrichs, C.M. Franz & D.J. Müller
FEBS Letters (2014) 588, 3639-3648. external page Read More

Stages and conformations of Tau repeat domain during aggregation and the question of neuronal toxicity

S. Kumar, K. Tepper, S. Kaniyappan, J. Biernat, S. Wegmann, E.M. Mandelkow, D.J. Müller & E. Mandelkow
Journal of Biological Chemistry (2014) 289, 20318-20332. external page Read More

Localizing chemical groups while imaging single native proteins by high-resolution AFM

M. Pfreundschuh, D. Alsteens, M. Hilbert, M.O. Steinmetz & D.J. Müller
Nano Letters (2014) 14, 2957-2964. external page Read More

Substrate-induced changes in the structural properties of LacY

T. Serdiuk, M.G. Madej, J. Sugihara, S. Kawamura, S.A. Mari, H.R. Kaback & D.J. Muller
Proc. Natl. Acad. Sci. USA (2014)  111, E1571-E1580. external page Read More

Multiparametric high-resolution imaging of native proteins by force-distance curve–based AFM

M. Pfreundschuh, D. Martinez-Martin, E. Mulvihill, S. Wegmann & D.J. Muller
Nature Protocols (2014) 9, 1113-1130. external page Read More

Dynamic coupling of ALCAM to the actin cortex strengthens cell adhesion to CD6

J. te Riet, J. Helenius, N. Strohmeyer, A. Cambi, C.G. Fidgor & D.J. Muller
Journal of Cell Science (2014) 127, 1595-1606. external page Read More

Single-cell force spectroscopy, an emerging tool to quantify cell adhesion to biomaterials

A. Taubenberger, D. Hutmacher & D.J. Muller
Tissue Engineering (2014) 20, 40-55. external page Read More

2013

Quantitative imaging of the electrostatic field and potential generated by a transmembrane protein at sub-nanometer resolution 

M. Pfreundschuh, U. Hensen & D.J. Muller
Nano Letters (2013) 13, 5585-5593. external page Read More

The peptide transporter DtpA populates two alternate conformations from which inhibitor binding promotes one

C. Bippes, L. Ge, M. Meury, D. Harder, Z. Ucurum, H. Daniel, D. Fotiadis & D.J. Muller
Proc. Natl. Acad. Sci. USA (2013) 110, E3978-3986. external page Read More

α_v-integrins are required for mechanotransduction in MDCK epithelial cells

T.P. Teräväinen, S.M. Myllymäki, J. Friedrichs, N. Strohmeyer, J.V. Moyano, C. Wu, K.S. Matlin, D.J. Muller & A. Manninen
PLoS One (2013) 8, e71485. Reexternal page ad More

Mechanistic explanation of different unfolding behaviors observed for transmembrane and soluble β-barrel proteins

U. Hensen & D.J. Muller
Structure (2013) 21, 1317-1324. external page Read More

Deciphering teneurin domains that facilitate cellular recognition, cell-cell adhesion and neurite outgrowth using AFM-based single-cell force spectroscopy

J. Beckmann, R. Schubert, R. Chiquet-Ehrismann & D.J. Muller
Nano Letters (2013) 13, 2937-2946. external page Read More

Single-molecule force spectroscopy of G-protein coupled receptors

M. Zocher, Ch. Bippes, C. Zhang & D.J. Muller
Chemical Society Reviews (2013) 42, 7801-7815. external page Read More

Multi-parametric imaging of biological systems by force-distance curve-based AFM

Y.F. Dufrene, D. Martínez-Martín, I. Medalsy, D. Alsteens & D.J. Muller
Nature Methods (2013) 10, 847-854. external page Read More

Nanomechanical properties of proteins and membranes depend on loading rate and electrostatic interactions

I. Medalsy & D.J. Muller
ACS NANO (2013) 7, 2642-2650. external page Read More

A practical guide to quantify cell adhesion using single-cell force spectroscopy

J. Friedrichs, K.R. Legate, R. Schubert, M. Bharadwaj, C. Werner, D.J. Muller & M. Benoit
Methods (2013) 60, 169–178. external page Read More

Wedged AFM-cantilevers for parallel plate cell mechanics

M.P. Stewart, A.W. Hodel, A. Spielhofer, C.J. Cattin, D.J. Müller & J. Helenius
Methods (2013) 60, 186–194. external page Read More

Quantifying cellular adhesion to covalently immobilized extracellular matrix proteins by single-cell force spectroscopy

J. Friedrichs, C. Werner & D.J. Muller
Methods in Molecular Biology (2013) 1046, 19-37. external page Read More

High-resolution imaging of 2D outer membrane protein F crystals by atomic force microscopy

D. Fotiadis & D.J. Müller
Methods in Molecular Biology (2013) 736, 461-474. external page Read More

Structural, kinetic, and mechanical differences between dark-state rhodopsin and opsin

S. Kawamura, M. Gerstung, L. Colozo, J. Helenius, N. Beerenwinkel, P.S.H. Park & D.J. Muller
Structure (2013) 21external page Read More

The fuzzy coat of pathological human Tau fibrils is a polyelectrolyte polymer brush

S. Wegmann, I. Medalsy, E. Mandelkow & D.J. Muller
Proc. Natl. Acad. Sci. USA (2013) 110, E313-321. external page Read More

2012

Cholesterol increases kinetic, energetic, and mechanical stability of the human β2 adrenergic receptor

M. Zocher, C. Zhang, G.F.S. Rassmussen, B.K. Kobilka & D.J. Muller
Proc. Natl. Acad. Sci. USA (2012) 109, E3463-3473. external page Read More

Out but not in: β-strands shape the unfolding pathway but not the refolding of the large transmembrane β-barrel protein FhuA

J. Thoma, P. Bosshart, M. Pfreundschuh & D.J. Muller
Structure (2012) 20, 2185-2190. external page Read More

Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase

C. Roos, M. Zocher, D.J. Muller, D. Munch, T. Schneider, H.G. Sahl, F. Scholz, J. Wachtveitl, Y. Ma, D. Proverbio, E. Henrich, V. Dötsch & F. Bernhard
Biochemical Biophysical Acta (2012) 1818, 3098-3106. external page Read More

Ligand-specific interactions modulate kinetic, energetic and mechanical properties of the human β2 adrenergic receptor

M. Zocher, J.J. Fung, B.K. Kobilka & D.J. Muller
Structure (2012) 20, 1391-1402. external page Read More

Structural, energetic and mechanical perturbations in a rhodopsin mutant that causes congenital stationary night blindness

S. Kawamura, A.L. Colozo, L. Ge, D.J. Muller & P.S.H. Park
Journal of Biological Chemistry (2012) 287, 21826-21835. external page Read More

Engineering rotor ring stoichiometries in ATP synthases

D. Pogoryelov, A.L. Klyszejko, G. Krasnoselska, E.M. Heller, V. Leone, J.D. Langer, J. Vonck, D.J. Muller, J.D. Faraldo-Gómez & T. Meier
Proc. Natl. Acad. Sci. USA (2012) 109, E1599-1608. external page Read More

Single-molecule force spectroscopy from nanodiscs – an assay to quantify folding, stability and interactions of native membrane proteins

M. Zocher, C. Roos, S. Wegmann, P. Bosshart, V. Doetsch, F. Bernhard & D.J. Muller
ACS NANO (2012) 6, 961-971. external page Read More

Tracking mechanics and volume of globular cells with atomic force microscopy using constant height clamp

M. Stewart, Y. Toyoda, A.A. Hyman & D.J. Muller
Nature Protocols (2012) 7, 143-154. external page Read More

KpOmpA a transmembrane protein anchoring the outer membrane of Klebsiella pneumoniae unfolds and refolds in response to tensile load

P. Bosshart, I. Iordanov, C. Garzon-Coral, P. Demange, A. Engel, A. Milon & D.J. Muller
Structure (2012) 20, 121-127. external page Read More

Investigating fibrillar aggregates of Tau protein by atomic force microscopy

S. Wegmann, D.J. Müller & E. Mandelkow
Methods in Molecular Biology (2012) 849, 169-183. external page Read More

2011

Gating of the MlotiK1 potassium channel involves large rearrangements of the cyclic nucleotide-binding domains

S.A. Mari, J. Pessoa, S.L. Altieri, U. Hensen, L. Thomas, J.H. Morais-Cabral & D.J. Muller
Proc. Natl. Acad. Sci. USA (2011) 108, 20802-20807. external page Read More

Structure and function of the glucose PTS transporter from Escherichia coli

J.-M. Jeckelmann, D. Harder, S.A. Mari, M. Meury, Z. Ucurum, D.J. Muller, B. Erni & D. Fotiadis
Journal of Structural Biology (2011) 176, 395-403. external page Read More

Locating an extracellular K⁺-dependent interaction site that modulates betaine-binding of the Na⁺-coupled betaine symporter BetP

L. Ge, C. Perez, I. Waclavska, C. Ziegler & D.J. Muller
Proc. Natl. Acad. Sci. USA (2011) 108, E890-898. external page Read More

Quantifying chemical and physical properties of native membrane proteins at molecular resolution by force-volume AFM

I. Medalsy, U. Hensen & D.J. Muller
Angewandte Chemie International Edition (2011) 50,12103-12108. external page Read More

Retinal pigment epithelium cell alignment on nanostructured collagen matrices

S. Ulbrich, J. Friedrichs, M. Valtink, S. Murovski, C.M. Franz, D.J. Muller, R.H.W. Funk & K. Engelmann
Cells Tissues Organs (2011) 194, 443–456. external page Read More

A size barrier limits protein diffusion at the cell surface to generate lipid-rich myelin-membrane sheets

S. Aggarwal, L. Yurlova, N. Snaidero, C. Reetz, S. Frey, J. Zimmermann, G. Pähler, A. Janshoff, J. Friedrichs, D.J. Muller, C. Goebel & M. Simons
Developmental Cell (2011) 21, 445-456. external page Read More

Seeing a molecular motor at work

W. Junge & D.J. Muller
Science (2011) 333, 704-705. external page Read More

Competing interactions stabilize pro- and anti-aggregant conformations of human Tau

S. Wegmann, J. Schöler, C.A. Bippes, E. Mandelkov & D.J. Muller
Journal of Biological Chemistry (2011) 286, 20512-20524. external page Read More

One β-hairpin after the other: Exploring refolding pathways and kinetics of the transmembrane β-barrel protein OmpG

M. Damaghi, S. Koester, C.A. Bippes, Ö. Yildiz & D.J. Muller
Angewandte Chemie International Edition (2011) 50, 7422-7424. external page Read More

Force generation: ATP-powered proteasomes pull the rope

Y.F. Dufrene & D.J. Muller
Current Biology (2011) 21, R427-R430. external page Read More

High-resolution atomic force microscopy and spectroscopy of native membrane proteins

Ch. Bippes & D.J. Muller
Reports on Progress in Physics (2011) 74, 086601. external page Read More

Force probing cell shape changes to molecular resolution

M.P. Stewart, Y. Toyoda, A.A. Hyman & D.J. Muller
Trends in Biochemical Sciences (2011) 36, 444-450. external page Read More

Atomic force microscopy: a nanoscopic window on the cell surface

D.J. Muller & Y.F. Dufrene
Trends in Cell Biology (2011) 21, 461-469. external page Read More

Force nanoscopy of living cells

D.J. Müller & Y.F. Dufrene
Current Biology (2011) 21, R212-R216. external page Read More

Five challenges to bringing single-molecule force spectroscopy into the living cell

Y.F. Dufrene, E. Evans, A. Engel, J. Helenius, H.E. Gaub & D.J. Müller
Nature Methods (2011) 8, 123-127. external page Read More

Combined activities of hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding

M.P. Stewart, J. Helenius, Y. Toyoda, S.P. Ramanathan, D.J. Müller & A.A. Hyman
Nature (2011) 469, 226–230. Rexternal page ead More

Studying collagen self-assembly by time-lapse high-resolution AFM

C. Franz & D.J. Müller
Methods in Molecular Biology (2011) 736, 97-107. external page Read More

2010

Planar cell polarity signalling coordinates epithelial organ formation by regulating cell adhesion properties in progenitor cells

P. Oteiza, M. Köppen, M. Krieg, S. Preibisch, E. Pulgar, C. Farias, C. Melo, D.J. Müller, M. Tada, S. Hartel, C.-P. Heisenberg & M.L. Concha
Development (2010) 137, 3459-68. external page Read More

Control of directed cell migration in vivo by membrane-to-cortex attachment

A. Diz-Muñoz, M. Krieg, M. Bergert, I. Ibarlucea-Benitez, D.J. Müller, E. Paluch, & C.P. Heisenberg
PLoS Biology (2010) 8, e1000544. external page Read More

Blood vessel lumen formation via electrostatic repulsion

B. Strilić, J. Eglinger, M. Krieg, M. Zeeb, J. Axnick, P. Babál, D.J. Müller & E. Lammert
Current Biology (2010) 20, 2003-2009. external page Read More

Conservation of molecular interactions stabilizing bovine and mouse rhodopsin

S. Kawamura, A.T. Colozo, D.J. Müller & P.S.H. Park
Biochemistry (2010) 49, 10412-10420. external page Read More

Human tau isoforms assemble into ribbon-like fibrils that display polymorphic structure and stability

S. Wegmann, Y.Y. Jung, S. Chinnathambi, E.M. Mandelkow, E. Mandelkow & D.J. Müller
Journal of Biological Chemistry (2010) 285, 27302-27313. external page Read More

A ”force buffer” protecting immunoglobulin titin

J.A. Nunes, U. Hensen, L. Ge, M. Lipinsky, J. Helenius, H. Grubmüller & D.J. Müller
Angewandte Chemie International Edition (2010) 49, 3528-3531. external page Read More

Assessing the structure and function of single biomolecules with scanning transmission electron and atomic force microscopes

S. Muller, D.J. Müller & A. Engel
Micron (2010) 42, 186-195. external page Read More

Dual energy landscape: The functional state of the outer-membrane β-barrel protein OmpG molds its unfolding energy landscape

M. Damaghi, K.T. Sapra, S. Köster, Ö. Yildiz, W. Kühlbrandt & D.J. Müller
Proteomics (2010) 10, 4151-4162. external page Read More

Quantifying interactions that switch the β-barrel membrane protein OmpG from the open to the closed state

M. Damaghi, C.A. Bippes, S. Köster, S.A. Mari, Ö. Yildiz, W. Kühlbrandt & D.J. Müller
Journal of Molecular Biology (2010) 397, 878-882. external page Read More

pH induced conformational change of the outer membrane protein OmpG reconstituted into native E. coli lipids

S.A. Mari, C.A. Bippes, S. Köster, Ö. Yildiz, W. Kühlbrandt & D.J. Müller
Journal of Molecular Biology (2010) 396, 610-616. external page Read More

Probing the interactions of carboxy-atractyloside and atractyloside with the yeast mitochondrial ADP/ATP carrier

A. Kedrov, A.M. Hellawell, A. Klosin, R.B. Broadhurst, E.R.S. Kunji & D.J. Müller
Structure (2010) 18, 39-46. external page Read More

Quantifying cellular adhesion to extracellular matrix components by single-cell force spectroscopy

J. Friedrichs, J. Helenius & D.J. Müller
Nature Protocols (2010) 5, 1353-1361. external page Read More

Stimulated single-cell force spectroscopy to quantify cell adhesion receptor crosstalk

J. Friedrichs, J. Helenius & D.J. Müller
Proteomics (2010) 10, 1455-1462. external page Read More

Effect of unlocking RGD-motifs in collagen I on pre-osteoblast adhesion and differentiation

A.V. Taubenberger, M.A. Woodruff, H. Bai, D.J. Müller & D. Hutmacher
Biomaterials (2010) 31, 2827-2835. external page Read More

Alignment and cell-matrix interactions of human corneal endothelial cells on nanostructured collagen type I matrices

S. Ulbrich, J. Friedrichs, M. Valtink, S. Murovski, C.M Franz, D.J. Müller, R.H.W. Funk & K. Engelmann
Investigative Opthamology & Visual Science (2010) 51, 6303-6310. external page Read More

Movement directionality in collective germ layer progenitor migration

Y. Arboleda-Estudillo, M. Krieg, J. Stuhmer, D.J. Müller & C.P. Heisenberg
Current Biology (2010) 20, 161-169. external page Read More

2009

One β-hairpin after the other: Exploring mechanical unfolding pathways of the transmembrane β-barrel protein OmpG

K.T. Sapra, M. Damaghi, S. Köster, Ö. Yildiz, W. Kühlbrandt & D.J. Müller

Angewandte Chemie International Edition (2009) 48, 8306-8308. external page Read More

Modulation of molecular interactions and function by rhodopsin palmitylation

P.S.H. Park, K.T. Sapra, B. Jastrzebska, T. Maeda, A. Maeda, W. Pulawski, M. Kono, J. Lem, R.K. Crouch, S. Filipek, D.J. Müller & K. Palczewski
Biochemistry (2009) 48, 4294-4304. external page Read More

The c13 ring from Bacillus TA2.A1 ATP synthase shows an extended diameter due to a special structural region

D. Matthies, L. Preiß, A.L. Klyszejko, D.J. Müller, G.M. Cook, J. Vonck & Th. Meier
Journal of Molecular Biology (2009) 388, 611-618. external page Read More

Substrate binding tunes conformational flexibility and kinetic stability of an amino acid transporter

C.A. Bippes, A. Zeltina, F. Casagrande, M. Ratera, M. Palacin, D.J. Müller & D. Fotiadis
Journal of Biological Chemistry (2009) 284, 18651-18663. external page Read More

Force probing surfaces of living cells to molecular resolution

D.J. Müller, J. Helenius, D. Alsteens & Y.F. Dufrene

Nature Chemical Biology (2009) 5, 383-390. external page Read More

Conformational adaptability of Redβ during DNA annealing and implications for its structural relationship with Rad52

A. Erler, S. Wegmann, C. Elie-Caille, R. Bradshaw, M. Maresca, R. Seidel, B. Habermann, D.J. Müller & F. Stewart
Journal of Molecular Biology (2009) 391, 586-598. external page Read More

New frontiers in atomic force microscopy: Analyzing interactions from single-molecules to cells

D.J. Müller, M. Krieg, D. Alsteens & Y.F. Dufrene
Current Opinion in Biotechnology (2009) 20, 4-13. external page Read More

Narrow-band UVB induced externalization of selected nuclear antigens in keratinocytes: Implications for lupus erythematosus pathogenesis

A. Reich, M. Meurer. A. Viehweg & D.J. Müller
Photochemistry and Photobiology (2009) 85, 1-7. external page Read More

Surface morphology and mechanical properties of fibroblasts from scleroderma patients

A. Reich, M. Meurer, B. Eckes, J. Friedrichs & D. J. Müller
Journal of Cellular and Molecular Medicine (2009) 13, 1644-1652. external page Read More

2008

TPA primes α2β1 integrins for cell adhesion

M. Tulla, J. Helenius, D.J. Müller & J. Heino
FEBS Letters (2008) 582, 3520-3524. external page Read More

A bond for a lifetime: Employing membrane nanotubes from living cells to determine receptor-ligand kinetics

M. Krieg, J. Helenius, C.-P. Heisenberg & D.J. Müller
Angewandte Chemie International Edition (2008) 47, 9775 –9777. external page Read More

AFM: A nanotool in membrane biology

D.J. Müller
Biochemistry (2008) 47, 7986-7998. external page Read More

High-throughput single-molecule force spectroscopy for membrane proteins

P.D Bosshart, F. Casagrande, P.L.T.M. Frederix, M. Ratera, C.A Bippes, D.J. Müller, M.l Palacin, A. Engel & D. Fotiadis
Nanotechnology (2008) 19, 384014. external page Read More

Fully automated single-molecule force spectroscopy for screening applications

J. Struckmeier, R. Wahl, M. Leuschner, J.M. Nunes, H. Janovjak, U. Geisler, G. Hofmann, T. Jähnke &D.J. Müller
Nanotechnology (2008) 19, 384020. external page Read More

Transducer binding establishes localized interactions that tune sensory rhodopsin II

D. Cisneros, L. Oberbarnscheidt, A. Pannier, J.P. Klare, J. Helenius, M. Engelhard, F. Oesterhelt & D.J. Müller
Structure (2008) 16, 1206-1213. external page Read More

Role of extracellular glutamic acids in the stability and energy landscape of bacteriorhodopsin

K.T. Sapra, J. Doehner, V. Renugolpalkrishan, E. Padros & D.J. Müller
Biophysical Journal (2008) 95, 3407-3418. external page Read More

Single-cell force spectroscopy

J. Helenius, C.P. Heisenberg, H.E. Gaub & D.J. Müller
Journal of Cell Science (2008) 121, 1785-1791. external page Read More

Galectin-3 regulates the kinetics of integrin α2β1 mediated adhesion to collagen-I and -IV

J. Friedrichs, A. Manninen, D.J. Müller & J. Helenius
Journal of Biological Chemistry (2008) 283, 32264-32272. external page Read More

Single-cell force spectroscopy reveals β1-integrin as a central molecule mediating adhesion of 32D-BCR-ABL cells to bone marrow stroma cells

F.A. Fierro, A. Taubenberger, P.-H. Puech, G. Ehninger, M. Bornhäuser, D.J. Müller & T. Illmer
Journal of Molecular Biology (2008) 377, 1082-1093.

Vertebrate membrane proteins: Structure, function and insights from biophysical approaches

D.J. Müller, N. Wu & K. Palczewski
Pharmacological Reviews (2008) 60, 43-78. external page Read More

Atomic force microscopy as a multifunctional molecular toolbox in nanobiotechnology

D.J. Müller & Y. Dufrene
Nature Nanotechnology (2008) 3, 261-269. [Commentary ‘Still feeling the force’ Nature Nanotechnology(2008) 3, 241] external page Read More

Quantifying adhesive and tensile cell properties determining germ layer organization during gastrulation

M. Krieg, Y. Arboleda, P.-H. Puech, J. Kaefer, F. Graener, D.J. Müller & C.P. Heisenberg
Nature Cell Biology (2008) 10, 429-436. [Commentary by J.B.A. Green ‘Sophistication of cell sorting’Nature Cell Biology (2008) 10, 375-377] external page Read More

Strategies to prepare and characterize native protein membranes by AFM

D.J. Müller & A. Engel
Current Opinion in Colloid and Interface Science (2008) 13, 338-350. Read More

Observing fibrillar assemblies on scrapie-infected cells

S. Wegmann, M. Miesbauer, K.F. Winklhofer, J. Tatzelt & D.J. Müller
Pflügers Archiv - European Journal of Physiology (2008) 456, 83-93. external page Read More

From valleys to ridges: Exploring the energy landscape of single membrane proteins

H. Janovjak, K.T. Sapra, A. Kedrov & D.J. Müller
ChemPhysChem (2008) 9, 954-956. external page Read More

An intermediate step in the evolution of ATPases - a hybrid FO-VO rotor in a bacterial Na+ F1FO ATP synthase

M. Fritz, A.L. Klyszejko, N. Morgner, J. Vonck, B. Brutschy, D.J. Müller, Th. Meier & V. Müller
FEBS Journal (2008) 275, 1999-2007. external page Read More

Folding and assembly of proteorhodopsin

A.L. Klyszejko, S. Shastri, S.A. Mari, H. Grubmüller, D.J. Müller & C. Glaubitz
Journal of Molecular Biology (2008) 376, 35-41. external page Read More

Mechanical properties of bovine rhodopsin and bacteriorhodopsin dictate energy landscape and guide conformational changes

K.T. Sapra, P.S.-H. Park, K. Palczewski & D.J. Müller
Langmuir (2008) 24, 1330-1337. external page Read More

Point mutations of membrane protein alter energy landscape and unfolding pathways

K.T. Sapra, P. Balasubramanian, D. Labudde, J. Bowie & D.J. Müller
Journal of Molecular Biology (2008) 376, 1076-1090. external page Read More

Examining the dynamic energy landscape of an antiporter upon inhibitor binding

A. Kedrov, M. Appel, H. Baumann, Ch. Ziegler & D.J. Müller
Journal of Molecular Biology (2008) 375, 1258-1266. external page Read More

2007

Straight GDP-tubulin protofilaments form in the presence of taxol

C. Elie-Caille, F. Severin, J. Helenius, J. Howard, D.J. Müller & A.A. Hyman
Current Biology (2007) 20, 1765-1770. external page Read More

Atomic force microscopy and spectroscopy of native membrane proteins

D.J. Müller & A. Engel
Nature Protocols (2007) 2, 2191-2197. external page Raed More

Studying integrin-mediated cell adhesion on the single-molecule level by using AFM force spectroscopy

C.M. Franz, A. Taubenberger, P.-H. Puech & D.J. Müller
Science STKE (2007) 406, pl5. Read More

Structural alterations provoked by narrow band ultraviolet B in immortalized keratinocytes: Assessment by atomic force microscopy

A. Reich, B. Lehmann, M. Meurer & D.J. Müller
Experimental Dermatology (2007) 16, 1007-1015. external page Read More

The oligomeric state of c rings from cyanobacterial F-ATP synthases varies from 13 to 15

D. Pogoryelov, C. Reichen, A. Klyszejko, R. Brunisholz, D.J. Müller, P. Dimroth & Th. Meier
Journal of Bacteriology (2007) 189, 5895-5902. external page Read More

Free energy of membrane protein unfolding derived from single-molecule force measurements

J. Preiner, H. Janovjak, C. Rankl, H. Knaus, D.A. Cisneros, A. Kedrov, F. Kienberger, D.J. Müller & P. Hinterdorfer
Biophysical Journal (2007) 93, 930-937. external page Read More

Galectin-3 and galectin-9 contribute to adhesion and cystogenesis in epithelial MDCK cells

J. Friedrichs, J. Torkko, J. Helenius, J. Füllekrug, D.J. Müller, K. Simons & A. Manninen
Journal of Biological Chemistry (2007) 282, 29375-29383. external page Read More

Cellular remodelling of individual collagen fibrils visualized by time-lapse AFM

J. Friedrichs, A. Taubenberger, C.M. Franz & D.J. Müller
Journal of Molecular Biology (2007) 372, 594-607. external page Read More

Stabilizing effect of Zn2+ in native bovine rhodopsin

P.S.-H. Park, K.T. Sapra, M. Koliński, S. Filipek, K. Palczewski & D.J. Müller
Journal of Biological Chemistry (2007) 282, 11377-11385. external page Read More

Transmembrane helices have rough energy surfaces

H. Janovjak, H. Knaus & D.J. Müller
Journal of the American Chemical Society (2007) 129, 246-247. external page Read More

Development of nanoscopic collagen matrices for biological and biotechnological applications

D.A. Cisneros, J. Friedrichs, A. Taubenberger, C. Franz & D.J. Müller
SMALL (2007) 3, 956-963. external page Read More

Revealing early steps of α2β1 integrin-mediated adhesions to collagen type I using single-cell force spectroscopy

A. Taubenberger, D.A. Cisneros, J. Friedrichs, P.-H. Puech, D.J. Müller & C.M. Franz
Molecular Biology of the Cell (2007) 18, 1634-1644. external page Read More

Aminosulfonate modulated pH induced conformational changes in Connexin26 hemichannels

J. Yu, Ch. Bippes. G. Hand, D.J. Müller & G. Sosinsky,
Journal of Biological Chemistry (2007) 282, 8895-8904. external page Read More

Detecting molecular interactions that stabilize, activate and guide ligand-binding of the sodium/proton antiporter MjNhaP1 from Methanococcus jannaschii

A. Kedrov, S. Wegmann, S. Smiths, P. Goswami, H. Baumann & D.J. Müller
Journal of Structural Biology (2007) 159, 290-301. external page Read More

Digital force-feedback for protein unfolding experiments using atomic force microscopy

Ch. Bippes, H. Janovjak, A. Kedrov & D.J. Müller
Nanotechnology (2007) 18, 1-7. doi:10.1088/0957-4484/18/4/044022 Rexternal page ead More

Deciphering molecular interactions of native membrane proteins by single-molecule force spectroscopy

A. Kedrov, H. Janovjak, K.T. Sapra & D.J. Müller
Annual Review of Biophysics and Biomolecular Structure (2007) 36, 233-260. external page Read More

A novel pattern recognition algorithm to classify membrane protein unfolding pathways with high-throughput single molecule force spectroscopy

A. Marisco, D. Labudde, T. Sapra, D.J. Müller & M. Schröder
Bioinformatics (2007) 23, e231-236. external page Read More

2006

Out and in: Simplifying membrane protein studies by AFM

D.J. Müller
Biophysical Journal (2006) 91, 3133-3134. external page Read More

Imaging and detecting molecular interactions of single transmembrane proteins

H. Janovjak, A. Kedrov, D.A. Cisneros, K.T. Sapra, J. Struckmeier & D.J. Müller
Neurobiology of Aging (2006) 27, 546-561. external page Read More

Characterizing folding, structure, molecular interactions and ligand gated activation of single sodium/proton antiporters

A. Kedrov & D.J. Müller
Naunyn-Schmiedeberg’s Archives of Pharmacology (2006) 372, 400-412. external page Read More

Hochauflösende Rasterkraftmikroskopie mikrobieller Membranen

D.J. Müller
Biospektrum (2006) 12, 483-485. external page Read More

Structure of the rhodopsin dimer: A working model for G-protein coupled receptors

D. Fotiadis, B. Jastrzebska, A. Philippsen, D.J. Müller, K. Palczewski & A. Engel
Current Opinion in Structural Biology (2006) 16, 252-259. external page Raed More

Single-molecule studies of membrane proteins

D.J. Müller, K.T. Sapra, S. Scheuring, A. Kedrov, P.L. Frederix, D. Fotiadis, & A. Engel
Current Opinion in Structural Biology (2006) 16, 489-495. Rexternal page ead More

Differentiating ligand and inhibitor interactions of a single antiporter

A. Kedrov, Ch. Ziegler & D.J. Müller
Journal of Molecular Biology (2006) 362, 925-932. external page Read More

A new approach to prepare membrane proteins for single molecule AFM imaging

D.A. Cisneros, D.J. Müller, S.M. Daud & J.H. Lakey
Angewandte Chemie International Edition (2006) 45, 3252-3256.

Analysis assistant for single-molecule force spectroscopy data on membrane proteins - MPTV

F. Müller, D.J. Müller & D. Labudde
Bioinformatics (2006) 22, 1796-1799. external page Read More

Detecting molecular interactions that stabilize native bovine rhodopsin

T. Sapra, P.S.H. Park, A. Engel, S. Filipek, D.J. Müller & K. Palzcewski
Journal of Molecular Biology (2006) 358, 255-269. external page Read More

Observing growth steps of collagen self-assembly by time-lapse high-resolution atomic force microscopy

D.A. Cisneros, C. Hung, C.M. Franz & D.J. Müller 
Journal of Structural Biology (2006) 154, 232-245. external page Read More

Bacteriorhodopsin folds into the membrane against an external force

M. Kessler, K. Gottschalk, H. Janovjak, D.J. Müller & H.E. Gaub
Journal of Molecular Biology (2006) 357, 644-654. Rexternal page ead More

A new technical approach to quantify cell adhesion forces by AFM

P.-H.Puech, K. Poole, D. Knebel & D.J. Müller
Ultramicroscopy (2006) 106, 637-644. external page Read More

Characterizing molecular interactions in different bacteriorhodopsin assemblies by single-molecule force spectroscopy

T. Sapra, H. Besir, D. Oesterhelt & D.J. Müller
Journal of Molecular Biology (2006) 355, 640-650. external page Read More

Direct measurement of single-molecule visco-elasticity in AFM force-extension measurements

Ch. Bippes, A. Humphris, D.J. Müller & H. Janovjak
European Biophysical Journal (2006) 35, 287-292. external page Read More

Observing folding pathways and kinetics of a single membrane protein

A. Kedrov, H. Janovjak, Ch. Ziegler, W. Kühlbrandt & D.J. Müller
Journal of Molecular Biology (2006) 355, 2-8.

2005

Zusammenhänge zwischen Oberflächenstruktur und funktionellem Zustand der Zellmembran von Krebszellen

K. Poole & D.J. Müller
Biospektrum (2005) 4, 465-466. external page Read More

Pattern recognition of single-molecule force spectroscopy data

M. Kuhn, H. Janovjak, M. Hubain, D. Labudde & D.J. Müller
Biophotonics International (2005) 12(8), 50-51. Rexternal page ead More

Microbial surfaces investigated by atomic force microscopy

Y. Dufrene & D.J. Müller
Methods in Microbiology (2005) 34, 163-197. external page Read More

Force microscopy and spectroscopy from single membrane proteins to entire cells

K. Poole & D.J. Müller
Imaging & Microscopy (2005) 3, 39-41. external page Read More

Wnt11 functions in gastrulation by controlling cell cohesion through Rab5c and E-cadherin

F. Ulrich, M. Krieg, E. Schötz, V. Link, I. Castanton, A. Taubenberger, D.J. Müller, P.-H. Puech & C.P. Heisenberg
Developmental Cell (2005) 9, 555-564. external page Read More

Analysing focal adhesion structure by AFM

C. Franz & D.J. Müller
Journal of Cell Science (2005) 118, 5315-5323. [Commentary by J. Bradbury ‘Taking the lid of focal adhesions’ Journal of Cell Science (2005) 118, e2104] Raed More

Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase

Th. Meier, J. Yu, Th. Raschle, F. Henzen, P. Dimroth & D.J. Müller
FEBS Journal (2005) 272, 5474-5483.

The c15 ring of the Spirulina platensis F-ATP synthase: F1/Fo symmetry mismatch is not obligatory

D. Pogoryelov, J. Yu, Th. Meier, J. Vonck, P. Dimroth & D.J. Müller
Embo reports (2005) 6, 1040-1044.

Measuring cell adhesion forces of primary gastrulating cells from zebrafish using atomic force microscopy

P.-H. Puech, A. Taubenberger, F. Ulrich, M. Krieg, D.J. Müller & C.P. Heisenberg
Journal of Cell Science (2005) 118, 4199-4206.

Locating ligand binding and activation of a single antiporter

A. Kedrov, M. Krieg, Ch. Ziegler, W. Kühlbrandt & D.J. Müller
Embo reports (2005) 6, 668-674.

Complex stability of single proteins explored by forced unfolding experiments

H. Janovjak, T.K. Sapra & D.J. Müller
Biophysical Journal (2005) 88, L37-L39.

Flexible, actin-based ridges co-localize with the beta1 integrin on the surface of melanoma cells

K. Poole & D.J. Müller
British Journal of Cancer (2005) 92, 1499-1505.

Automated alignment and pattern recognition of single-molecule force spectroscopy data

M. Kuhn, H. Janovjak, M. Hubain & D.J. Müller
Journal of Microscopy (2005) 218, 125-132.

Probing different origins of potential barriers stabilizing the membrane proteins halorhodopsin and bacteriorhodopsin

D. Cisneros, D. Oesterhelt & D.J. Müller
Structure (2005) 13, 235-242. [Commentary by G. Chin ‘Deconstructing membrane proteins’ Science (2005) 307, 1172]

Dynamic response of single bacteriorhodopsins, determined by single-molecule force modulation spectroscopy

H. Janovjak, D.J. Müller & A. Humphris
Biophysical Journal (2005) 88, 1423-1431. [Commentary by J. van Noort ‘Unravelling bacteriorhodopsin’ Biophysical Journal (2005) 88, 763-764]

Extended range of force loading rates for AFM single-molecule experiments

H. Janovjak, J. Struckmeier & D.J. Müller
European Biophysical Journal (2005) 34, 91-96.

Molecular-scale topographic cues induce the orientation and directional movement of fibroblasts on two-dimensional collagen surfaces

K. Poole, K. Khairy, J. Friedrichs, C. Franz, D. Cisneros, J. Howard & D.J. Müller
Journal of Molecular Biology (2005) 349, 380-386.

Cell surface ridges characterized by advanced light and atomic force microscopy

A. Sharma, K. Anderson & D.J. Müller
FEBS Letters (2005) 579, 2001-2008.

2004

Atomic force microscopy of biological samples

P. Frederix, B.W. Hoogenboom, D. Fotiadis, D.J. Müller & A. Engel
MRS Bulletin (2004) 29, 449-455.

Assembly of collagen into microribbons: effects of pH and electrolytes

F. Jiang, H. Hörber, J. Howard & D.J. Müller
Journal of Structural Biology (2004) 148, 268-278.

The effect of raft lipid depletion on microvilli formation in MDCK cells, visualized by atomic force microscopy

K. Poole, D. Meder, K. Simons & D.J. Müller
FEBS Letters (2004) 565, 53-58.

Creating nanoscopic collagen matrices using atomic force microscopy

F. Jiang, K. Khairy, K. Poole, J. Howard & D.J. Müller
Microscopy Research Technique (2004) 64, 435-440.

Probing the energy landscape of the membrane protein bacteriorhodopsin

H. Janovjak, J. Struckmeier, M. Hubain, A. Kedrov, M. Kessler & D.J. Müller
Structure (2004) 12, 871-879.

Controlled unfolding and refolding of a single sodium-proton antiporter

A. Kedrov, Ch. Ziegler, H. Janovjak, W. Kühlbrandt & D.J. Müller
Journal of Molecular Biology (2004) 340, 1143-1152.

2003

Atomic force bio-analytics

P. Frederix, T. Akiyama, U. Staufer, Ch. Gerber, D. Fotiadis, D.J. Müller & A. Engel
Current Opinion in Chemical Biology (2003) 7, 641-647.

Fourteen protomers compose the oligomer III of the proton-rotor in spinach chloroplast ATP-synthase

H. Seelert, N. Dencher & D.J. Müller
Journal of Molecular Biology (2003) 333, 374-344.

Temperature dependent unfolding of bacteriorhodopsin

H. Janovjak, M. Kessler, D. Oesterhelt, H. Gaub & D.J. Müller
EMBO Journal (2003) 22, 5220-5229.

Observing the growth of individual actin filaments in cell extracts by time-lapse atomic force microscopy

T. Lehto, M. Miaczynska, M. Zerial, D.J. Müller & F. Severin
FEBS Letters (2003) 551, 25-28.

Determining molecular forces that stabilize human aquaporin-1

C. Moeller, D. Fotiadis, K. Suda, A. Engel, M. Kessler & D.J. Müller
Journal of Structural Biology (2003) 142, 369-378.

Observing membrane protein diffusion at subnanometer resolution

D. J. Müller, A. Engel, Th. Meier, U. Matthey, P. Dimroth & K. Suda
Journal of Molecular Biology (2003) 327, 925-930.

2002

Controlled nanomanipulation of biological specimens with the atomic force microscope

D. Fotiadis, S. Scheuring, S.A. Müller, A. Engel & D.J. Müller
Micron (2002) 33, 385-397.

Conformations, flexibility, and interactions observed on individual membrane proteins by atomic force microscopy

D.J. Müller & A. Engel
Methods in Cell Biology (2002) 68, 257-299.

Observing structure function and assembly of single proteins by AFM

D.J. Müller, H. Janovjak, T. Lehto, L. Kuerschner & K. Anderson
Progress in Biophysics and Molecular Biology (2002) 71, 1-43.

Biomolecular imaging using atomic force microscopy

D.J. Müller & K. Anderson
Trends in Biotechnology (2002) 20, S45-S49.

Sampling effects influence the heights measured with atomic force microscopy

J.B. Heymann, C. Moeller & D.J. Müller
Journal of Microscopy (2002) 205,43-51.

Stability of bacteriorhodopsin α-helices and loops analyzed by single molecule force spectroscopy

D.J. Müller, M. Kessler, F. Oesterhelt, C. Moeller, D. Oesterhelt & H. Gaub
Biophysical Journal (2002) 83, 3578-3588.

Conformational changes in surface structures of isolated Connexin26 gap junctions

D.J. Müller, G.M. Hand, A. Engel & G.E. Sosinsky
EMBO Journal (2002) 21, 3598-3607.

Identification and structure of a putative Ca²⁺-binding domain at the C terminus of AQP1

D. Fotiadis, K. Suda, P. Tittmann, P. Jenö, A. Philippsen, D.J. Müller, H. Gross & A. Engel
Journal of Molecular Biology (2002) 318, 1381-1394.

Imaging the electrostatic potential of transmembrane channels: Atomic probe microscopy of OmpF porin

A. Philippsen, W. Im, A. Engel, T. Schirmer, B. Roux & D.J. Müller
Biophysical Journal (2002) 82, 1667-1676.

Sampling the conformational space of membrane protein surfaces with the AFM

S. Scheuring, D.J. Müller, H. Stahlberg, H.-A. Engel & A. Engel
European Biophysical Journal (2002) 31, 172-178.

Isolation and characterization of gap junctions from tissue culture cells

G.M. Hand, D.J. Müller, B. Nicholson, A. Engel & G.E. Sosinsky
Journal of Molecular Biology (2002) 315, 587-

2001

Single proteins observed by atomic force microscopy

S. Scheuring, D. Fotiadis, C. Möller, S. Müller, A. Engel & D.J. Müller
Single Molecule (2001) 2, 59-67.

The central plug in the reconstituted undecameric c cylinder of a bacterial ATP synthase consists of phospholipids

Th. Meier, U. Matthey, F. Henzen, P. Dimroth, & D.J. Müller
FEBS Letters (2001) 505, 353-356.

Subunits constrain stoichiometry of ATP synthase rotor

D.J. Müller, N. Dencher, Th. Meier, P. Dimroth, H. Stahlberg, K. Suda, A. Engel, H. Seelert & U. Matthey
FEBS Letters (2001) 504, 219-222.

Bacterial sodium ATP synthase has an undecameric motor

H. Stahlberg, D.J. Müller, K. Suda, D. Fotiadis, A. Engel, Th. Meier, U. Matthey & P. Dimroth
EMBO Reports (2001) 2, 229-233.

From images to interactions: High-resolution phase imaging in tapping-mode atomic force microscopy

M. Stark, C. Möller, D.J. Müller & R. Guckenberger
Biophysical Journal (2001) 80, 3009-3018.

2000

Observing proteins at work with the atomic force microscope

A. Engel & D.J. Müller
Nature Structural Biology (2000) 7, 715-718.

Reversible loss of crystallinity during photobleaching purple membrane

C. Möller, G. Büldt, N.A. Dencher, A. Engel & D.J. Müller
Journal of Molecular Biology (2000) 301, 869-879.

The surface topography of lens MIP supports dual functions

D. Fotiadis, L. Hasler, D.J. Müller, H. Stahlberg, J. Kistler & A. Engel
Journal of Molecular Biology (2000) 300, 779-789.

Atomic force microscopy of native purple membrane

D.J. Müller, J.B. Heymann, F. Oesterhelt, C. Möller, H. Gaub, G. Büldt & A. Engel
Biochemical Biophysical Acta (2000) 1460, 27-38.

Conformational changes, flexibilities and intramolecular forces observed on individual proteins using AFM

D.J. Müller, D. Fotiadis, C. Möller, S. Scheuring & A. Engel
Single Molecule (2000) 1, 115-118.

Conformations of the rhodopsin third cytoplasmic loop grafted onto bacteriorhodopsin

J.B. Heymann, M. Pfeiffer, V. Hildebrandt, D. Fotiadis, B. de Groot, R. Kabak, A. Engel, D. Oesterhelt & D.J. Müller
Structure (2000) 8, 643-653.

Proton powered turbine of a plant motor

H. Seelert, A. Poetsch, N. Dencher, A. Engel, H. Stahlberg & D.J. Müller
Nature (2000) 405, 418-419.

Unfolding pathways of individual bacteriorhodopsins

F. Oesterhelt, D. Oesterhelt, M. Pfeiffer, A. Engel, H. Gaub & D.J. Müller
Science (2000) 288, 143-146. [Commentary by J.G. Forbes & G.H. Lorimer ‘Unravelling a membrane protein’ Science (2000) 288, 63-64]