Pipetting Helper Imaging Lid – PHIL - 2021
Open-source personal pipetting robots with live-cell incubation and microscopy compatibility
Overview
- personal small desktop liquid handling robot
- small, light, cheap
- fast self-assembly from 3D printed and commercial parts
- long-term live cell culture media exchange, immunostainings, etc
- compatible with microscope stage-top use during time-lapse microscopy
- user-friendly control software
Reference
If you use PHIL please cite the following paper:
Dettinger P, Kull T, Arekatla G, Ahmed N, Zhang Y, Schneiter F, Wehling A, Schirmacher D, Kawamura S, Loeffler D and Schroeder T (2021)
external page Personal open-source pipetting robots with stage-top live-cell incubation and imaging capability.
bioRxiv, 2021.07.04.448641
Download Files
Current version
Download PHIL_Complete_Resources_210716.zip (ZIP, 47.2 MB)
2021.07.16: Added companies with international shipping options to «Order_List.xlsx» in PHIL_Complete_Resources.zip
Previous versions
2021.07.05: Download PHIL_Complete_Resources - .zip (ZIP, 47.2 MB)
ZIP file contents
PHIL operation
PHIL_Operation_Instructions.pdf: Complete instructions for using PHIL
PHIL_GUI_210602.m: Executable Matlab script for PHIL gui
PHIL production
- Order_List.xlsx: Complete parts list for all required parts
- PHIL_Print_Instructions.pdf: Full print settings of all parts
- 3D print files as described below
- PHIL_Assembly_Instructions.pdf: Full instructions for PHIL assembly
- PHIL_Arduino_MEGA_14Pump_210216.ino: Complete Arduino sketch for PHIL operation. Requires Accelstepper library installed
3D print files
Individual components are encoded by the individual STL files below. You can use these to print them individually.
All required components for a PHIL robot have been grouped into 10 sets for easy simultaneous printing of several components in one print session.
- Set_1.stl
Body_VB1.stl: Main body of PHIL
Body_Top_VB1.stl: Frame for mounting Z-axis stepper motors
Elbow_Left_VB1.stl: Left actuator arm for mounting limit switches
Elbow_Right_VB1.stl: Right actuator arm for mounting limit switches
Hands_VB1.stl: Left and right actuator arm
Tube_Plug_VB1.stl: Tube holder for PHIL body
- Set_2.stl
Arm_Plate_VB1.stl: Stepper motor mount for left and right arms
Body_Bottom_VB1.stl: Base for main body of PHIL
- Set_3_100infill.stl
LR_Arm_100Infill.stl: Left and right arms for PHIL. To be printed at 100% infill
- Set_4.stl
Chamber _VB1.stl: Incubator chamber
2 x Screw_VB1.stl: Screw drives for z-axis
- Set_5.stl
Chamber_Lid_VB1.stl: Lid for incubator chamber
USB_Back_VB1.stl: USB cable mounting bracket, back
USB_Front_VB1.stl: USB cable mounting bracket, front
Handle_VB1.stl: Handle for power control box
Pin_Cup_V1.stl: Pipet holder for normal wells
(Pin_Cup_V1.stl can be replaced with Pin_Cup_Deep_Well_VB1.stl when working with deep well multiwell plates - see instructions)
- Set_6.stl
Power_Box_Left_VB1.stl: Left half of power control box
- Set_7.stl
Power_Box_Right_VB1.stl: Right half of power control box
- Set_8.stl
Arduino_Plate_VB1.stl: Plate for mounting arduinos
- Set_9.stl
Mount_1x_31.stl: 6x mounting brackets for peristaltic pump
- Set_10.stl
Wheel_1x_31.stl: 6x rotors for peristaltic pump
The following files are not required for a PHIL, but help the production:
- tube_bender_top_v2.stl: Tube bending press outer body
- tube_bender_bot_v2.stl: Tube bending press inner frame
- 85mm_Tube_cutter_v2.stl: Jig for cutting 85mm segment of peristaltic pump tubing
License
Copyright (c) 2021 Schroeder Lab
Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:
The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.