HD-MEAs to characterize human induced pluripotent-stem-cell-derived neurons
We used high-density microelectrode-arrays (HD-MEAs) to study physiology of healthy and diseased human induced pluripotent-stem-cell-derived neurons across scales, from network through single-neuron to subcellular features.
Recent advances in the field of cellular reprogramming have opened a route to studying the fundamental mechanisms underlying common neurological disorders. High-density microelectrode-arrays (HD-MEAs) provide unprecedented means to study neuronal physiology at different scales, ranging from network through single-neuron to subcellular features. In the paper "Electrophysiological phenotype characterization of human iPSC‐derived neuronal cell lines by means of high‐density microelectrode arrays" (Advanced Biology 2021, Article 2000223) by S. Ronchi et al., HD-MEAs were used in vitro to characterize and compare human induced pluripotent-stem-cell-derived dopaminergic and motor neurons, including isogenic neuronal lines modeling Parkinson’s disease (PD) and amyotrophic lateral sclerosis (ALS). Reproducible electrophysiological network, single-cell and subcellular metrics, were used for phenotype characterization and drug testing. Metrics, such as burst shape and axonal velocity, enabled the distinction of healthy and diseased neurons.
An image of the paper also was selected for the front cover of the journal.
external page Advanced Biology (previously Advanced Biosystems) covers life-science research across multiple disciplines including but not limited to biology, chemistry, physics, medical science and computer science, applied to biologically relevant systems at all scales, ranging from molecular to whole-organism level and beyond.
