RedLibs
RedLibs is an algorithm that allows for the rational design of smart combinatorial libraries for pathway optimization thereby minimizing the use of experimental resources.
RedLibs is free to use. The source code can be downloaded here:
Source Code Download
Photograph of violacein (black), mCherry (red) producing, and negative control (white) E. coli colonies sorted as RedLibs logo pattern. Many thanks to Telma Lopes from the Single Cell Facility for operating the cell sorter for this unconventional application.
Published Application Examples
Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort.
M. Jeschek, D. Gerngross, S. Panke
Nature Communications, 7 (2016): 11163. external page DOI
Overcoming the membrane barrier: Recruitment of γ-glutamyl transferase for intracellular release of metabolic cargo from peptide vectors.
T. Kuenzl, M. Sroka, P. Srivastava, P. Herdewijn, P. Marlière, S. Panke
Metabolic Engineering, 39 (2017): 60-70. external page DOI
Efficient engineering of chromosomal ribosome binding site libraries in mismatch repair proficient Escherichia coli.
S. Oesterle, D. Gerngross, S. Schmitt, T. M. Roberts, S. Panke
Scientific Reports, 7 (2017). external page DOI