Publications

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external pageEpistasis arises from shifting the rate-limiting step during enzyme evolution of a β-lactamase.
Fröhlich C*, Bunzel HA*, Mulholland AJ, Kamp MWvd, Johnsen PJ, Leiros HKS, Tokuriki N, Nat. Catal. 2024.
Selection of a Promiscuous Minimalist cAMP Phosphodiesterase from a Library of De Novo Designed Proteins.
Schnettler JD, Wang MS, Gantz M, Bunzel HA, Karas C, Hollfelder F, Hecht MH, Nat. Chem. accepted.
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external pageElectric fields determine carbapenemase activity in class A β-lactamases. Jabeen H, Beer M, Spencer J, Kamp MWvd, Bunzel HA, Mulholland AJ, bioRxiv 2023.
external pageComment on: “Computer Simulations Reveal an Entirely Entropic Activation Barrier for the Chemical Step in a Designer Enzyme”
Lear A, Anderson JLR, Hilvert D, Arcus VL, Kamp MWvd, Bunzel HA, Mulholland AJ. ACS Catal 2023.
external pageAn expandable, modular de novo protein platform for precision redox engineering.
Hutchins GH, Noble CEM, Bunzel HA, Williams C, Dubiel P, Yadav SKN, Molinaro PM, Barringer R, Blackburn H, Hardy BJ, Parnell AE, Landau C, Race PR, Oliver TAA, Koder RL, Crump MP, Schaffitzel C, Oliveira ASF, Mulholland AJ, Anderson JLR. PNAS 2023.
external pageIn vitro generated antibodies guide thermostable ADDomer nanoparticle design for nasal vaccination and passive immunization against SARS-CoV-2.
Buzas D, Bunzel HA, Staufer O, Milodowski EJ, Edmunds GL, Bufton JC, Mateo BVV, Yadav SKN, Gupta K, Fletcher C, Williamson MK, Harrison A, Borucu U, Capin J, Francis O, Balchin G, Hall S, Vega MV, Durbesson F, Vincentelli R, Roe, J Wooldridge L, Burt R, Anderson JLR, Mulholland AJ, Bristol UNCOVER Group, Hare J, Bailey M, Davidson AD, Finn A, Morgan D, Mann J, Spatz J, Garzoni F, Schaffitzel C, Berger I. Antib. Ther. 2023.
external pagePhotovoltaic enzymes by design and evolution.
Bunzel HA, Smith JA, Oliver TAA, Jones MR, Mulholland AJ, Anderson JLR. bioRxiv 2022.
external pageEvolution of dynamical networks enhances catalysis in a designer enzyme.
Bunzel HA, Anderson JLR, Hilvert D, Arcus VL, Kamp MWvd, Mulholland AJ. Nat Chem 2021.
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external pageDesigning better enzymes: Insights from directed evolution.
Bunzel HA, Anderson JLR, Mulholland AJ. Curr Opin Struct Biol 2021.
external pageRigidifying a de novo enzyme increases activity and induces a negative activation heat capacity.
Hindson SA*, Bunzel HA*, Frank B*, Svistunenko DA, Williams C, van der Kamp MW, Mulholland AJ, Pudney CR, Anderson JLR. ACS Catal 2021.
external pageEfficient Lewis acid catalysis of an abiological reaction in a de novo protein scaffold.
Basler S, Studer S, Zou Y, Mori T, Ota Y, Camus A, Bunzel HA, Helgeson RC, Houk KN, Jiménez-Osés G, Hilvert D. Nat Chem 2021.
external pageBeneficial substrate partitioning boosts non-aqueous catalysis in de novo enzyme-alginate beads.
Stenner R*, Bunzel HA*, Mulholland AJ, Anderson JLR. bioRxiv 2021.
external pageHow directed evolution reshapes the energy landscape in an enzyme to boost catalysis.
Otten R*, Pádua RAP*, Bunzel HA*, Nguyen V, Pitsawong W, Patterson M, Sui S, Perry SL, Cohen AE, Hilvert D, Kern D. Science 2020.
external pageContribution of Oxyanion Stabilization to Kemp Eliminase Efficiency.
Kries H*, Bloch JS*, Bunzel HA*, Pinkas DM, Hilvert D. ACS Catal 2020.external page
external pageEmergence of a Negative Activation Heat Capacity during Evolution of a Designed Enzyme .
Bunzel HA, Kries H, Marchetti L, Zeymer C, Mittl P, Mulholland A, Hilvert D. J Am Chem Soc 2019.
external pageSpeeding up enzyme discovery and engineering with ultrahigh-throughput methods.
Bunzel HA*, Garrabou X*, Pott M*, Hilvert D. Curr Opin Struct Biol 2018.