Publications
- external page Epistasis arises from shifting the rate-limiting step during enzyme evolution of a β-lactamase.
Fröhlich C*, Bunzel HA*, Mulholland AJ, Kamp MWvd, Johnsen PJ, Leiros HKS, Tokuriki N, Nat. Catal. 2024. - external page Selection of a Promiscuous Minimalist cAMP Phosphodiesterase from a Library of De Novo Designed Proteins.
Schnettler JD, Wang MS, Gantz M, Bunzel HA, Karas C, Hollfelder F, Hecht MH, Nat. Chem. 2024.
- external page Electric fields determine carbapenemase activity in class A β-lactamases. Jabeen H, Beer M, Spencer J, Kamp MWvd, Bunzel HA, Mulholland AJ, ACS Catal. 20243.
- external page Comment on: “Computer Simulations Reveal an Entirely Entropic Activation Barrier for the Chemical Step in a Designer Enzyme”
Lear A, Anderson JLR, Hilvert D, Arcus VL, Kamp MWvd, Bunzel HA, Mulholland AJ. ACS Catal 2023. - external page An expandable, modular de novo protein platform for precision redox engineering.
Hutchins GH, Noble CEM, Bunzel HA, Williams C, Dubiel P, Yadav SKN, Molinaro PM, Barringer R, Blackburn H, Hardy BJ, Parnell AE, Landau C, Race PR, Oliver TAA, Koder RL, Crump MP, Schaffitzel C, Oliveira ASF, Mulholland AJ, Anderson JLR. PNAS 2023. - external page In vitro generated antibodies guide thermostable ADDomer nanoparticle design for nasal vaccination and passive immunization against SARS-CoV-2.
Buzas D, Bunzel HA, Staufer O, Milodowski EJ, Edmunds GL, Bufton JC, Mateo BVV, Yadav SKN, Gupta K, Fletcher C, Williamson MK, Harrison A, Borucu U, Capin J, Francis O, Balchin G, Hall S, Vega MV, Durbesson F, Vincentelli R, Roe, J Wooldridge L, Burt R, Anderson JLR, Mulholland AJ, Bristol UNCOVER Group, Hare J, Bailey M, Davidson AD, Finn A, Morgan D, Mann J, Spatz J, Garzoni F, Schaffitzel C, Berger I. Antib. Ther. 2023.
- external page Photovoltaic enzymes by design and evolution.
Bunzel HA, Smith JA, Oliver TAA, Jones MR, Mulholland AJ, Anderson JLR. bioRxiv 2022. - external page Evolution of dynamical networks enhances catalysis in a designer enzyme.
Bunzel HA, Anderson JLR, Hilvert D, Arcus VL, Kamp MWvd, Mulholland AJ. Nat Chem 2021.
external page Nature Portfolio Highlight - external page Designing better enzymes: Insights from directed evolution.
Bunzel HA, Anderson JLR, Mulholland AJ. Curr Opin Struct Biol 2021. - external page Rigidifying a de novo enzyme increases activity and induces a negative activation heat capacity.
Hindson SA*, Bunzel HA*, Frank B*, Svistunenko DA, Williams C, van der Kamp MW, Mulholland AJ, Pudney CR, Anderson JLR. ACS Catal 2021. - external page Efficient Lewis acid catalysis of an abiological reaction in a de novo protein scaffold.
Basler S, Studer S, Zou Y, Mori T, Ota Y, Camus A, Bunzel HA, Helgeson RC, Houk KN, Jiménez-Osés G, Hilvert D. Nat Chem 2021. - external page Beneficial substrate partitioning boosts non-aqueous catalysis in de novo enzyme-alginate beads.
Stenner R*, Bunzel HA*, Mulholland AJ, Anderson JLR. bioRxiv 2021. - external page How directed evolution reshapes the energy landscape in an enzyme to boost catalysis.
Otten R*, Pádua RAP*, Bunzel HA*, Nguyen V, Pitsawong W, Patterson M, Sui S, Perry SL, Cohen AE, Hilvert D, Kern D. Science 2020. - external page Contribution of Oxyanion Stabilization to Kemp Eliminase Efficiency.
Kries H*, Bloch JS*, Bunzel HA*, Pinkas DM, Hilvert D. ACS Catal 2020.external page - external page Emergence of a Negative Activation Heat Capacity during Evolution of a Designed Enzyme .
Bunzel HA, Kries H, Marchetti L, Zeymer C, Mittl P, Mulholland A, Hilvert D. J Am Chem Soc 2019. - external page Speeding up enzyme discovery and engineering with ultrahigh-throughput methods.
Bunzel HA*, Garrabou X*, Pott M*, Hilvert D. Curr Opin Struct Biol 2018.