Dr. Nicolas Huguenin-Dezot

Dr.  Nicolas Huguenin-Dezot

Dr. Nicolas Huguenin-Dezot

Staff of Professorship for Bioprocess

ETH Zürich

Bioverfahrenstechnik, Panke

BSS G 35.5

Klingelbergstrasse 48

4056 Basel

Switzerland

Research

The focus of our group is to study atypical modes of DNA replication and exploit their potential as biotechnological tools. We use a various range of structural biology techniques (X-ray crystallography, NMR and cryo-EM) and synthetic biology methods (directed evolution and unnatural amino acid incorporation) to study these fascinating processes. Ultimately, our group is interested in developing new ways to evolve proteins and endow them with new functions. These newly evolved proteins could find various applications (from the development/production of innovative drugs to new chemical agents or enzymes/organism helping to address environmental issues) that will help solve some of the challenges that our society is facing in the 21st century.

Open positions

Our group is looking for motivated Master’s, PhD students and PostDocs who would like to make valuable research contributions to our research field. Please contact Dr. Huguenin-Dezot directly (nicolas.huguenin-dezotATbsse.ethz.ch) with your CV and a motivation letter.

Curriculum Vitae

Group members

Honours and Awards

Patents

Publications

external pageDiscovery and Genetic Code Expansion of a Polyethylene Terephthalate (PET) Hydrolase from the Human Saliva Metagenome for the Degradation and Bio-Functionalization of PET. Eiamthong, B., Meesawat, P., Wongsatit, T., Jitdee, J., Sangsri, R., Patchsung, M., Aphicho, K., Suraritdechachai, S., Huguenin-Dezot, N., Tang, S., Suginta, W., Paosawatyanyong, B., Babu, M., Chin, J. W., Pakotiprapha, D., Bhanthumnavin, W., Uttamapinant, C. Ang. Chem. Int. Ed. (2022).

external pageMechanism-based traps enable protease and hydrolase substrate discovery. Shan Tang, Adam T. Beattie, Lucie Kafkova, Gianluca Petris, Nicolas Huguenin-Dezot, Marc Fiedler, Matthew Freeman & Jason W. Chin. Nature (2022).

 

external pageTrapping biosynthetic acyl-enzyme intermediates with encoded 2,3-diaminopropionic acid. Huguenin-Dezot, N., Alonzo, D., Heberlig, G., Mohan, M., Nguyen, D. P., Dornan, M., Body, C., Schmeing, M., Chin, J. W. Nature (2019).

 

external pageMitotic phosphorylation regulates Hsp72 spindle localization by uncoupling ATP binding from substrate release. Mukherjee, M., Sabir, S., O'Regan, L., Sampson, J., Richards, M. W., Huguenin-Dezot, N., Ault, J. R., Chin, J. W., Zhuravleva, A., Fry, A. M. & Bayliss, R. Science Signaling (2018).

 

external pageIntracellular antibody signalling is regulated by phosphorylation of the Fc receptor TRIM21. Dickson, C., Fletcher, A. J., Vaysburd, M., Yang, J-C., Mallery, D. L., Zeng, J., Johnson, C. M., McLaughlin, S. H., Skehel, M., Maslen, S., Cruickshank, J., Huguenin-Dezot, N., Chin, J. W., Neuhaus, D., James, L. C. Elife (2018).

 

external pageMitotic spindle association of TACC3 requires Aurora-A-dependent stabilisation of a cryptic a-helix. Burgess, S., Mukherjee, M., Sabir, S., Joseph, N., Gutiérrez-Caballero, C., Richards, M. W., Huguenin-Dezot, N., Chin, J. W., Kennedy, E. J., Pfuhl, M., Royle, S. J., Gergely, F., Bayliss R. The EMBO Journal (2018).

 

external pageBiosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing. Zhang, M. S., Brunner, S. F., Huguenin-Dezot, N., Liang, A. D., Schmied, W. H., Rogerson, D. T., Chin, J. W. Nature Methods (2017).

 

external pageSynthesis of Isomeric Phosphoubiquitin Chains Reveals that Phosphorylation Controls Deubiquitinase Activity and Specificity. Huguenin-Dezot, N., De Cesare, V., Peltier, J., Knebel, A., Kristaryianto, Y. A., Rogerson, D. T., Kulathu, Y., Trost, M. & Chin, J. W. Cell Rep (2016).

 

external pageEfficient genetic encoding of phosphoserine and its nonhydrolyzable analog. Rogerson, D. T., Sachdeva, A., Wang, K., Haq, T., Kazlauskaite, A., Hancock, S. M., Huguenin-Dezot, N., Muqit, M. M. K., Fry, A. M., Bayliss, R. & Chin, J. W. Nat. Chem. Biol. (2015).

 

external pageSite-Specific Traceless Coupling of Potent Cytotoxic Drugs to Recombinant Antibodies for Pharmacodelivery. Casi, G., Huguenin-Dezot, N., Zuberbühler, K., Scheuermann, J. & Neri, D. J. Am. Chem. Soc. (2012)

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