Dr. Nicolas Huguenin-Dezot
Dr. Nicolas Huguenin-Dezot
Staff of Professorship for Bioprocess
Research
The focus of our group is to study atypical modes of DNA replication and exploit their potential as biotechnological tools. We use a various range of structural biology techniques (X-ray crystallography, NMR and cryo-EM) and synthetic biology methods (directed evolution and unnatural amino acid incorporation) to study these fascinating processes. Ultimately, our group is interested in developing new ways to evolve proteins and endow them with new functions. These newly evolved proteins could find various applications (from the development/production of innovative drugs to new chemical agents or enzymes/organism helping to address environmental issues) that will help solve some of the challenges that our society is facing in the 21st century.
Open positions
Our group is looking for motivated Master’s, PhD students and PostDocs who would like to make valuable research contributions to our research field. Please contact Dr. Huguenin-Dezot directly (nicolas.huguenin-dezotATbsse.ethz.ch) with your CV and a motivation letter.
Curriculum Vitae
Group members
Honours and Awards
Patents
Publications
external pageDiscovery and Genetic Code Expansion of a Polyethylene Terephthalate (PET) Hydrolase from the Human Saliva Metagenome for the Degradation and Bio-Functionalization of PETcall_made. Eiamthong, B., Meesawat, P., Wongsatit, T., Jitdee, J., Sangsri, R., Patchsung, M., Aphicho, K., Suraritdechachai, S., Huguenin-Dezot, N., Tang, S., Suginta, W., Paosawatyanyong, B., Babu, M., Chin, J. W., Pakotiprapha, D., Bhanthumnavin, W., Uttamapinant, C. Ang. Chem. Int. Ed. (2022).
external pageMechanism-based traps enable protease and hydrolase substrate discoverycall_made. Shan Tang, Adam T. Beattie, Lucie Kafkova, Gianluca Petris, Nicolas Huguenin-Dezot, Marc Fiedler, Matthew Freeman & Jason W. Chin. Nature (2022).
external pageTrapping biosynthetic acyl-enzyme intermediates with encoded 2,3-diaminopropionic acidcall_made. Huguenin-Dezot, N., Alonzo, D., Heberlig, G., Mohan, M., Nguyen, D. P., Dornan, M., Body, C., Schmeing, M., Chin, J. W. Nature (2019).
external pageMitotic phosphorylation regulates Hsp72 spindle localization by uncoupling ATP binding from substrate releasecall_made. Mukherjee, M., Sabir, S., O'Regan, L., Sampson, J., Richards, M. W., Huguenin-Dezot, N., Ault, J. R., Chin, J. W., Zhuravleva, A., Fry, A. M. & Bayliss, R. Science Signaling (2018).
external pageIntracellular antibody signalling is regulated by phosphorylation of the Fc receptor TRIM21call_made. Dickson, C., Fletcher, A. J., Vaysburd, M., Yang, J-C., Mallery, D. L., Zeng, J., Johnson, C. M., McLaughlin, S. H., Skehel, M., Maslen, S., Cruickshank, J., Huguenin-Dezot, N., Chin, J. W., Neuhaus, D., James, L. C. Elife (2018).
external pageMitotic spindle association of TACC3 requires Aurora-A-dependent stabilisation of a cryptic a-helixcall_made. Burgess, S., Mukherjee, M., Sabir, S., Joseph, N., Gutiérrez-Caballero, C., Richards, M. W., Huguenin-Dezot, N., Chin, J. W., Kennedy, E. J., Pfuhl, M., Royle, S. J., Gergely, F., Bayliss R. The EMBO Journal (2018).
external pageBiosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencingcall_made. Zhang, M. S., Brunner, S. F., Huguenin-Dezot, N., Liang, A. D., Schmied, W. H., Rogerson, D. T., Chin, J. W. Nature Methods (2017).
external pageSynthesis of Isomeric Phosphoubiquitin Chains Reveals that Phosphorylation Controls Deubiquitinase Activity and Specificitycall_made. Huguenin-Dezot, N., De Cesare, V., Peltier, J., Knebel, A., Kristaryianto, Y. A., Rogerson, D. T., Kulathu, Y., Trost, M. & Chin, J. W. Cell Rep (2016).
external pageEfficient genetic encoding of phosphoserine and its nonhydrolyzable analogcall_made. Rogerson, D. T., Sachdeva, A., Wang, K., Haq, T., Kazlauskaite, A., Hancock, S. M., Huguenin-Dezot, N., Muqit, M. M. K., Fry, A. M., Bayliss, R. & Chin, J. W. Nat. Chem. Biol. (2015).
external pageSite-Specific Traceless Coupling of Potent Cytotoxic Drugs to Recombinant Antibodies for Pharmacodeliverycall_made. Casi, G., Huguenin-Dezot, N., Zuberbühler, K., Scheuermann, J. & Neri, D. J. Am. Chem. Soc. (2012)