Equipment
The Single Cell Facility provides access to seven flow cytometry analyzers and seven sorters.
Analyzers - are used for sample acquisition and analysis only. We have three BD Fortessa (two of them equipped with HTS system), three BC Cytoflex (one integrated in the lab automation facility) and one spectral Cytek Aurora. They can be operated by researchers themselves upon mandatory training. All analyzers can be used for the analysis of both BSL1 and BSL2 samples. To reserve the instruments please use our OpenIRIS booking system.
Sorters - are used for cells and particle separation. We have seven different cell sorters: BD FACS Aria Fusion, BD FACS Aria III, BD FACSDiscover S8, BD FACSMelody, BC Cytoflex SRT, Sony SH800 and Sony MA900. The Sony SH800, Sony MA900, BC Cytoflex SRT and BD FACSMelody can be operated by researchers themselves upon mandatory training. To reserve the instruments please use our OpenIRIS booking system. The usage of BD FACS Aria Fusion, BD FACS Aria III and BD FACSDiscover S8 is operator assisted only. To reserve these instruments please send an e-mail to . All of our sorters, except for the BD FACS Melody, are suitable to work with BSL2 samples.
Our facility has three flow cytometry experts on staff: Mariangela, Chiara and Ola. If you want to know more about our equipment or how to make a reservation, contact our staff at .
Available laser lines on the different instruments
Each instrument is equipped with several lasers. Please check the table below to find the instrument that suits your experimental needs. To find out more details about each instrument's optical configuration, see the individual technical specifications.
Optical filters
Filters are used to separate specific wavelenght band of the emitted light. They can be exchanged to adapt the instrument configuration to the researchers needs. Please contact the Flow staff for the full lists of the all filters available in the facility.
Quality control
To ensure consistent and reliable data, quality control checks are performed regularly scheduled on all instruments.