Cytek Aurora

The Cytek Aurora is a full-spectrum flow cytometer that captures complete emission profiles using 64 fluorescence detectors across 5 lasers, enabling the creation of high-parameter multicolor panels. By recording full spectral signatures rather than relying on peak intensities, it can separate fluorochromes withsimilar spectra, allowing the detection of both established and novel dyes.

The system also helps reduce the impact of cellular autofluorescence, such as in primary tissues, improving the resolution of dim signals. Its APD-based detectors provide increased sensitivity in the red range, supporting flexible panel design across a broad spectral space.

Optical Configuration

Standard filter configuration of the instrument:

Lasers available: 355 nm, 405 nm, 488 nm, 561 nm, 640 nm

Total number of detectors: 64

Technical Information:

Supported carrier types: 

- 96 WP flat, U, V bottom

- 96 deep well

- 5 ml tubes in 40-tube rack

Dead Volumes:

- 10 ul for 96 flat bottom plates

- 5 ul for all other supported plate types

Sample flow rates:

- tube mode between 15 ul/min  and 60 ul/min

- plate mode between 15 ul/min  and 100 ul/min

Data acquisition rate: 35000 events/sec