BD Fortessa Analyzer
We have three BD LSR Fortessa SORP (Special Order Research Product) instruments in our facility. Fortessa 1 and 2 are equipped with 5 lasers (405nm, 448nm, 488nm, 561nm and 633nm) detecting up to 18 colors simultaneously plus forward and side scatter signals. These analyzers are located in U1.44 and therefore only BSL-1 samples can be acquired.
Fortessa 3 has an additional 355nm laser and since it is placed in a BSL-2 lab (U1.43), also BSL-2 level samples can also be analyzed.
The optical filters of all analyzers can be exchanged to configure the instrument appropriately for the individual combinations of fluorochromes.
Optical Configuration:
Technical Information:
• We have installed the Forward Scatter (FSC) PMT option that can lower the threshold for size measurements in Fortessa 1 and 2. This is most often used for the analysis of smaller particles such as microbial cells.
• Only 5mL tubes with the reference 352054 from BD can be used on these instruments.
• Fortessa 1 and 3 are equipped with an HTS loader (see below) to enable automated sample acquisition from 96 or 384 well plates.
HTS Information:
The High Throughput Sampler (HTS) provides rapid, fully automated sample acquisition of 96 or 384 well plates.