Dr. Hannah Minas

In my work, I focus on a group of large biosynthetic assembly lines, called non-ribosomal peptide synthetases. These enzymes have an intrinsic modularity which can be used to construct new, artificial synthetases by recombining modules from different synthetases. To improve the functionality of our designed assembly lines, I set up a high throughput cloning workflow whose data obtained by high throughput mass spectrometric analysis is then used to train a machine learning model. In addition to the functionality of the assembly lines themselves, their products are also assessed for bioactivity and promising scaffolds are further diversified to obtain antibiotic lead structures.