Mitigating the impact of limited cell resources on mammalian synthetic circuits

Synthetic gene circuits transferred into mammalian cells often show large discrepancies between intended and actual behaviour. Part of this problem is that gene expression depends on finite cellular resources. The Control Theory & Systems Biology Lab led by Mustafa Khammash and collaborators now experimentally characterised the effects of limited cellular resources caused by transient gene expression in mammalian cells and provided solutions to alleviate them.