Dr. Daniel Gerngross
Dr. Daniel Gerngross
Staff of D-BSSE Laboratory Automation Facility
Research
Within the external page NCCR Molecular Systems Engineering I'm investigating design rules for synthetic operons. While progresses in synthetic biology are leading to more and more efficient answers to the question “How to write DNA?”, the question of “What to write into the DNA?” remains challenging. I aim to answer this question in regard to bacterial polycistronic operon systems. Such operons, in which multiple genes are expressed from only one promoter, are an important functional unit in bacteria. In order to gain enough knowledge to engineer future multi-protein systems I am deploying a top-down approach, where high-throughput DNA assembly helps me to construct large numbers of variants and multiplexed multilevel assays provide me quantitative information about the system parameters or DNA copy-numbers, mRNA amounts, and protein level and activity.
Furthermore, I'm interested in developing bioinformatic tools that allow efficient screening for fine tuned protein expression levels in synthetic systems, e.g. the algorithm for generating smart RBS libraries:
Curriculum Vitae
Education
Teaching
Publications
Chemo-enzymatic cascades to produce cycloalkenes from bio-based resources.
Shuke Wu, Yi Zhou, Daniel Gerngross, Markus Jeschek & Thomas R. Ward
Nature Communications, (2019).
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Industrializing a Bacterial Strain for L-Serine Production through Translation Initiation Optimization.
Maja Rennig, Hemanshu Mundhada, Gossa G. Wordofa, Daniel Gerngross, Tune Wulff, Andreas Worberg, Alex T. Nielsen, Morten H. H. Nørholm
ACS Synthetic Biology, (2019).
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Efficient engineering of chromosomal ribosome binding site libraries in mismatch repair proficient Escherichia coli.
Sabine Oesterle, Daniel Gerngross, Steven Schmitt, Tania M. Roberts, Sven Panke
Scientific Reports, (2017).
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Combinatorial pathway optimization for streamlined metabolic engineering.
Markus Jeschek, Daniel Gerngross, Sven Panke
Current opinion in biotechnology, (2017).
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Synthetic Biology for Cell-Free Biosynthesis: Fundamentals of Designing Novel In Vitro Multi-Enzyme Reaction Networks.
Gaspar Morgado, Daniel Gerngross, Tania M. Roberts, Sven Panke
Advances in Biochemical Engineering/Biotechnology, (2016).
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Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort.
Markus Jeschek, Daniel Gerngross, Sven Panke
Nature Communications, 7 (2016): 11163.
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