Tracking different cell types in human brain organoids
Stem-cell derived organoids have become a promising model for studying human organ development and modelling diseases. In an earlier study, researchers from the Quantitative Developmental Biology lab led by Barbara Treutlein and colleagues showed how different cell types emerge in human cerebral organoids. They now present a new method that shows how cells transition from their pluripotent state to form multiple cell types and regions and hence, directly measures lineage relationships.
The new method developed by Zhisong He, Ashley Maynard, Akanksha Jain and other researchers in the Treutlein-lab together with Gray Camp from the Institute of Molecular and Clinical Ophthalmology, University of Basel, is named: iTRACER, a novel barcoding and scarring system that allows to track lineage relationships in developing human cerebral organoids.
Publication details:
He, Z, A Maynard, A Jain, T Gerber, R Petri, H-C Lin, M Santel, K Ly, J-S D, L Sidow, F Sanchis, S M J Jansen, S Riesenberg, J G Camp, and B Treutlein. Lineage recording in human cerebral organoids. Nature Methods, external page https://doi.org/10.1038/s41592-021-01344-8
The external page journal's news & views featured this study.
Find 2019-publication by Kanton, S et al., external page https://doi.org/10.1038/s41586-019-1654-9
Learn about the Quantitative Developmental Biology lab led by Barbara Treutlein.