Improved method for engineering therapeutic and diagnostic proteins on yeast
Surface proteins on yeast cells play a key role in biomedical applications. The process involves producing modified proteins on the yeast surface and selecting cells that exhibit favourable behaviour, such as binding to a target molecule. A study in ACS Synthetic Biology led by researchers around Michael Nash now presents a new method that equips yeast cells with specific genes that control the amount of protein produced on the cell surface.
“The ability to control protein abundance on the yeast cell surface will benefit protein engineering and directed evolution for broad classes of therapeutic and diagnostic proteins.”Michael Nash, Lab for Molecular Engineering of Synthetic Systems, D-BSSE / ETH Zurich and Department of Chemistry, University of Basel.
Find original publication in ACS Synthetic Biology:
Lopez-Morales, J, R Vanella, G Kovacevic, M Sá Santos, and M Nash (2023) external pageTitrating avidity of yeast-displayed proteins using a transcriptional regulator. ACS Synthetic Biology 12, https://doi.org/10.1021/acssynbio.2c00351
Find external pagenews article issued by the Swiss Nanoscience Institute.
Learn about the external pageLab for Molecular Engineering of Synthetic Systems led by Michael Nash.